Eur. J. Entomol. 106 (4): 471-476, 2009 | DOI: 10.14411/eje.2009.059

Down-regulation of gene expression between the diapause initiation and maintenance phases of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

George D. YOCUM, Joseph P. RINEHART, Marnie L. LARSON
USDA-ARS Red River Valley Agricultural Research Center, Biosciences Research Laboratory, 1605 Albrecht Boulevard, Fargo, ND 58105, USA; e-mail: george.yocum@ars.usda.gov

The diapause initiation and maintenance phases of the Colorado potato beetle, Leptinotarsa decemlineata, were screened. Eight transcripts were found to be downregulated as the beetles enter the diapause maintenance phase of diapause development after day 15 postemergence. These transcripts were also expressed in early nondiapausing adults. Using BlastX, the transcripts were placed into six broad categories: regulatory (serpin), structural (apidermin), protease (serine protease), retinol binding protein (CRALBP), carbohydrate metabolism (ß-glucosidase, ß-mannosidase, and cellulose II), and unknown function.

Keywords: Chrysomelidae, Coleoptera, diapause, gene expression

Received: May 18, 2009; Revised: July 20, 2009; Accepted: July 20, 2009; Published: November 20, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
YOCUM, G.D., RINEHART, J.P., & LARSON, M.L. (2009). Down-regulation of gene expression between the diapause initiation and maintenance phases of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). EJE, 106(4), 471-476. doi: 10.14411/eje.2009.059
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. ALTSCHUL S.F., MADDEN T.L., SCHAFFER A.A., ZHANG J., ZHANG Z., MILLER W. & LIPMAN D.J. 1997: Gapped Blast and PSIBlast: a new generation of protein database search programs. Nucl. Acids Res. 25: 3389-3402 Go to original source...
    2. ANTAO C.M. & MALCATA F.X. 2005: Plant serine proteases: biochemical, physiological and molecular features. Plant Physiol. Biochem. 43: 637-650 Go to original source...
    3. BAKER M.B. & PORTER A.H. 2008: Use of sperm precedence to infer the overwintering cost of insecticide resistance in the Colorado potato beetle. Agric. For. Entomol. 10: 181-187 Go to original source...
    4. BOSSE T.C. & VEERMAN A. 1996: Involvement of vitamin A in FERRO D.N. 1985: Pest status and control strategies of the Colorado potato beetle. In Ferro D.N. & Voss R.N. (eds): Proceedings of the Symposium on the Colorado Potato Beetle, 17th International Congress of Entomology. Massachusetts Agricultural Experimental Station Research Bulletin 704, pp. 1-8
    5. FRANZL S., ACKERMANN I. & NAHRSTEDT A. 1989: Purification and characterization of a ss-glucosidase (linamarase) from the haemolymph of Zygaena trifolii Esper, 1783. (Insecta, Lepidoptera). Experientia 45: 712-718 Go to original source...
    6. GAO N., VON SCHANTZ M., FOSTER R.G. & HARDIE J. 1999: The putative brain photoperiodic photoreceptor in the vetch aphid, Megoura viciae. J. Insect Physiol. 45: 1011-1019 Go to original source...
    7. GAUTHIER N.L., HOFMASTER R.N. & SEMAL M. 1981: History of Colorado potato beetle control. In Lashomb J.H. & Casagrande R.A. (eds): Advances in Potato Pest Management. Hutchinson Ross, Stroudsburg, PA, pp. 13-33
    8. GOOPTU B. & LOMAS D.A. 2008: Polymers and inflammation: disease mechanisms of the serpinopathies. J. Exp. Med. 205: 1529-1534 Go to original source...
    9. DE GREGORIO E., HAN S.-J., LEE W.-J., BAEK M.-J., OSAKI T., KAWABATA S.-I., LEE B.L., IWANAGA S., LEMAITRE B. & BREY P.T. 2002: An immune-responsive serpin regulates the melanization cascade in Drosophila. Dev. Cell 3: 581-592 Go to original source...
    10. HACHEM J.-P., HOUBEN E., CRUMRINE D., MAN M.-Q., SCHURER N., ROELANDT T., CHOI E.H., UCHIDA Y., BROWN B.E., FEINGOLD K.R. & ELIAS P.M. 2006: Serine proteases signaling of epidermal permeability barrier homeostasis. J. Invest. Dermatol. 126: 2074-2086 Go to original source...
    11. VAN HOUTEN Y.M. & VEERMAN A. 1990: Photoperiodism and thermoperiodism in the predatory mite Amblyseius potentillae are probably based on the same mechanism. J. Comp. Physiol. (A) 167: 201-209 Go to original source...
    12. KOEPPE J.K., FUCHS M., CHEN T.T., HUNT L.-M., KOVALICK G.E. & BRIERS T. 1985: The role of juvenile hormone in reproduction. In Kerkut G.A. & Gilbert L.I. (eds): Comprehensive Insect Physiology, Biochemistry and Pharmacology. Vol. 8. Pergamon Press, Oxford, pp. 165-203 Go to original source...
    13. KOSTAL V. 2006: Eco-physiological phases of insect diapause. J. Insect Physiol. 52: 113-127 Go to original source...
    14. KUCHARSKI R., MALESZKA J. & MALESZKA R. 2007: Novel cuticular proteins revealed by the honey bee genome. Insect Biochem. Mol. Biol. 37: 128-134 Go to original source...
    15. LAW R.H.P., ZHANG Q., MCGOWAN S., BUCKLE A., SILVERMAN G.A., WONG W., ROSADO C.J., LANGENDORF C.G., PIKE R.N., BIRD P.I. & WHISSTOCK J.C. 2006: An overview of the serpin superfamily. Genome Biol. 7: 216 Go to original source...
    16. LI H.-M., BUCZKOWSKI G., MITTAPALLI O., XIE J., WU J., WESTERMAN R., SCHEMERHORN B.J., MURDOCK L.L. & PITTENDRIGH B.P. 2008: Transcriptomic profiles of Drosophila melathe photoperiodic induction of diapause in the spider mite Tetranychus urticae is demonstrated by rearing an albino mutant on a semi-synthetic diet with and without ss-carotene or vitamin A. Physiol. Entomol. 21: 188-192 Go to original source...
    17. CLARET J. & VOLKOFF N. 1992: Vitamin A is essential for two processes involved in the photoperiodic reaction in Pieris brassicae. J. Insect Physiol. 38: 569-574 Go to original source...
    18. DENLINGER D.L. 2008: Why study diapause? Entomol. Res. 38: 1-9 Go to original source...
    19. EPPO 2006: Distribution maps of quarantine pests for Europe, Leptinotarsa decemlineata. http://pqr.eppo.org/datas/ LPTNDE/LPTNDE.pdf
    20. FERREIRA C., TORRES B.D. & TERRA W.R. 1998: Substrate specificities of midgut ss-glycosidases from insects of different orders. Comp. Biochem. Physiol. (B) 119: 219-225. nogaster third instar larval midgut and response to oxidative stress. Insect Mol. Biol. 17: 325-339 Go to original source...
    21. LUKE C.J., PAK S.C., ASKEW Y.S., NAVIGLIA T.L., ASKEW D.J., NOBAR S.M., VETICA A.C., LONG O.S., WATKINS S.C., STOLZ D.B., BARSTEAD R.J., MOULDER G.L., BROMME D. & SILVERMAN G.A. 2007: An intracellular serpin regulates necrosis by inhibiting the induction and sequelae of lysosomal injury. Cell 130: 1108-1119 Go to original source...
    22. NOY N. 2000: Retinoid-binding proteins: mediators of retinoid action. Biochem. J. 348: 481-495 Go to original source...
    23. OVERMEER W.P.J., NELIS H.J.C.F., DE LEENHEER A.P., CALIS J.N.M. & VEERMAN A. 1989: Effect of diet on the photoperiodic induction of diapause in three species of predatory mite, Amblyseius potentillae, A. cucumeris, and Typhlodromus pyri. Exp. Appl. Acarol. 7: 281-287. Go to original source...
    24. PEFEROEN M., HUYBRECHTS R. & DE LOOF A. 1981: Longevity and fecundity in the Colorado potato beetle, Leptinotarsa decemlineata. Entomol. Exp. Appl. 29: 321-329 Go to original source...
    25. ROBERTS T.H. & HEJGAARD J. 2008: Serpins in plants and green algae. Func. Integr. Genomics 8: 1-27 Go to original source...
    26. ROBICH R.M. & DENLINGER D.L. 2005: Diapause in the mosquito Culex pipiens evokes a metabolic switch from blood feeding to sugar gluttony. Proc. Natl. Acad. Sci. USA 102: 15912-15917 Go to original source...
    27. SCHERFER C., TANG H., KAMBRIS Z., LHOCINE N., HASHIMOTO C. & LEMAITRE B. 2008: Drosphila serpin-28D regulates hemolymph phenoloxidase activity and adult pigment. Dev. Biol. 323: 189-196 Go to original source...
    28. SCRIVENER A.M., WATANABE H. & NODA H. 1997: Diet and carbohydrate digestion in the yellow-spotted longicorn beetle, Psacothea hilaris. J. Insect Physiol. 43: 1039-1052 Go to original source...
    29. SILVERMAN G.A., BIRD P.I., CARRELL R.W., CHURCH F.C., COUGHLIN P.B., GETTINS P.G.W., IRVING J.A., LOMAS D.A., LUKE C.J., MOYER R.W., PEMBERTON P.A., REMOLDO'DONNELL E., SALVESEN G.S., TRAVIS J. & WHISSTOCK J.C. 2001: The serpins are an expanding superfamily of structurally similar but functionally diverse proteins. J. Biol. Chem. 276: 33293-33296 Go to original source...
    30. TAUBER M.J. & TAUBER C.A. 1973: Insect phenology; criteria for analyzing dormancy and for forecasting postdiapause development and reproduction in the field. Search Agric. 11: 1-16
    31. TAUBER M.J., TAUBER C.A. & MASAKI S. 1986: Seasonal Adaptations in Insects. Oxford Press, Oxford, 411 pp
    32. TAUBER M.J., TAUBER C.A., OBRYCKI J.J., GOLLANDS B. & WRIGHT R.J. 1988: Geographical variation in responses to photoperiod and temperature by Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) during and after dormancy. Ann. Entomol. Soc. Am. 81: 764-773 Go to original source...
    33. TERRA W.R. & FERREIRA C. 1994: Insect digestive enzymes: properties, compartmentalization and function. Comp. Biochem. Physiol. (B) 109: 1-62 Go to original source...
    34. VEERMAN A., SLAGT M.E., ALDERLIESTS M.F.J. & VEENENDAAL R.L. 1985: Photoperiodic induction of diapause in an insect is vitamin A dependent. Experientia 41: 1194-1195 Go to original source...
    35. VERHAGEN A.M., SILKE J., EKERT P.G., PAKUSCH M., KAUFMANN H., CONNOLLY L.M., DAY C.L., TIKOO A., BURKE R., WROBEL C., MORITZ R.L., SIMPSON R.J. & VAUX D.L. 2002: HtrA2 promotes cell death through its serine protease activity and its ability to antagonize inhibitor of apoptosis proteins. J. Biol. Chem. 277: 445-454 Go to original source...
    36. WATANABE H. & TOKUDA G. 2001: Animal cellulases. Cell. Mol. Life Sci. 58: 1167-1178 Go to original source...
    37. WEBER D.C. & FERRO D.N. 1994: Colorado potato beetle: Diverse life history poses challenge to management. In Zehnder G.W., Powelson M.L., Jansson R.K. & Raman K.V. (eds): Advances in Potato Pest Biology and Management. APS Press, St. Paul, Minnesota, pp. 54-70
    38. WILLIS J.H., ICONOMIDOU V.A., SMITH R.F. & HAMODRAKAS S.J., 2005: Cuticular proteins. In Gilbert L.I., Iatrow K. & Gill S.S. (eds): Comprehensive Molecular Insect Science. Vol. 4. Elsevier Oxford, UK, pp. 79-110 Go to original source...
    39. YOCUM G.D. 2001: Differential expression of two HSP70 transcripts in response to cold shock, thermoperiod, and adult diapause in the Colorado potato beetle. J. Insect Physiol. 47: 1139-1145 Go to original source...
    40. YOCUM G.D. 2003: Isolation and characterization of three diapause-associated transcripts from the Colorado potato beetle, Leptinotarsa decemlineata. J. Insect Physiol. 49: 161-169 Go to original source...
    41. YOCUM G.D., COUDRON T.A. & BRANDT S.L. 2006: Differential gene expression in Perillus bioculatus nymphs fed a suboptimal artificial diet. J. Insect Physiol. 52: 586-592 Go to original source...
    42. YOCUM G.D., RINEHART J.P., CHIRUMAMILLA-CHAPARA A. & LARSON M.L. 2009: Characterization of gene expression patterns during the initiation and maintenance phases of diapause in the Colorado potato beetle, Leptinotarsa decemlineata. J. Insect Physiol. 55: 32-39 Go to original source...
    43. ZHANG J., GOYER C. & PELLETIER Y. 2008: Environmental stresses induce the expression of putative glycine-rich insect cuticular protein genes in adult Leptinotarsa decemlineata (Say). Insect Mol. Biol. 17: 209-216 Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content