EJE, vol. 112 (2015), issue 4

A new look at the nature of insect juvenile hormone with particular reference to studies carried out in the Czech Republic


Eur. J. Entomol. 112 (4): 567-590, 2015 | DOI: 10.14411/eje.2015.073

This article is a comprehensive summary of the 50-year history of physiological investigations in the Czech Republic into the mode of action of the corpus allatum hormone (CAH) in insects, which is commonly known as the juvenile hormone (JH). During this period 4000 synthetic JH- mimetic bioanalogues were tested. The sesquiterpenoid epoxy-homofarnesoate (JH-I), which is generally thought to be the true JH of insects, is an excretory product of the male colleterial gland, not an insect hormone. There are two principal hormones produced by the insect neuroendocrine system: activation hormone (AH) produced by neurosecretory cells in the brain and JH secreted...

Parasitoids of the lime leaf miner Phyllonorycter issikii (Lepidoptera: Gracillariidae) recorded throughout the area it recently colonized

Levente SZŐCS, Melika GEORGE, Csaba THURÓCZY, György CSÓKA

Eur. J. Entomol. 112 (4): 591-598, 2015 | DOI: 10.14411/eje.2015.101

Phyllonorycter issikii (Lepidoptera: Gracillariidae) has recently spread over a considerable part of the Eurasian continent. So far, in Europe, unlike in Russia, it has not significantly affected its food plant (Tilia cordata), but its effect may increase over time. The regulating potential of associated parasitoid natural enemies is still low, which probably allows further leaf miner range expansion and population growth. This species is potentially capable of inflicting considerable damage on its host. Therefore, it is important to study its natural enemies, especially their oviposition behaviour and biology. In this paper, we summarize...

First chromosomal study of Mantophasmatodea: Karyotype of Karoophasma biedouwense (Austrophasmatidae)


Eur. J. Entomol. 112 (4): 599-605, 2015 | DOI: 10.14411/eje.2015.093

We have investigated for the first time the chromosomes of Karoophasma biedouwense, a species belonging to the Mantophasmatodea, a recently discovered order of carnivorous insects. Our study has revealed that males of this species display testes with numerous seminal tubes (follicles), as in other Polyneoptera, and short tubular seminal vesicles embedded in a utricular gland. The karyotype consists of 2n = 12A + X monocentric and biarmed, meta/submetacentric chromosomes (fundamental number of arms: FN = 26) with blocks of heterochromatin around centromeres. The autosomes are classified into two size groups, one represented by a single,...

Facultative parthenogenesis in the burrowing mayfly, Ephoron eophilum (Ephemeroptera: Polymitarcyidae) with an extremely short alate stage

Kazuki SEKINÉ, Koji TOJO, Yeon Jae BAE

Eur. J. Entomol. 112 (4): 606-612, 2015 | DOI: 10.14411/eje.2015.074

Facultative parthenogenesis is important for mayflies with short alate stages because females are able to reproduce without mating. We studied facultative parthenogenesis in Ephoron eophilum, a mayfly with an extremely short alate stage. We examined the survival rates of embryos from unfertilized eggs, in addition to investigating the number of chromosomes in parthenogenetic offspring and the mode of inheritance by nuclear genetic analyses using Exon-Primed Intron-Crossing markers. The survival rate of thelytokous embryos was 0-70.2% (16.7 ± 26.7%, mean ± S.D.). Sixteen chromosomes were present throughout most of the mitotic metaphase...

Origins and genetic diversity of the ragweed beetles, Ophraella communa (Coleoptera: Chrysomelidae), that were introduced into Italy and Japan based on an analysis of mitochondrial DNA sequence data

Yudai NISHIDE, Yuya FUKANO, Hayato DOI, Toshiyuki SATOH, Hiroki INOUE, Marco BORIANI

Eur. J. Entomol. 112 (4): 613-618, 2015 | DOI: 10.14411/eje.2015.086

Ophraella communa (Coleoptera: Chrysomelidae) is an oligophagous herbivorous beetle that feeds on Ambrosia artemisiifolia. It is native to North America, but was accidentally introduced into Japan in 1995 and Europe in 2013. We analyzed partial DNA sequences of the mitochondrial cytochrome oxidase II gene for O. communa collected from 29 locations in the United States, Japan and Italy. Overall, the results of our analyses indicate that the introduced Japanese populations have lower genetic variation than the native populations. The sequences for the Italian specimens did not share haplotypes with Japanese specimens. These results...

Bacillus in the guts of honey bees (Apis mellifera; Hymenoptera: Apidae) mediate changes in amylase values

Miao WANG, Wen-Zheng ZHAO, Hong XU, Zheng-Wei WANG, Shao-Yu HE

Eur. J. Entomol. 112 (4): 619-624, 2015 | DOI: 10.14411/eje.2015.095

Amylase is one of three main enzymes involved in the breakdown and utilization of macromolecules. In honey bees (Apis mellifera), amylase was traditionally believed to originate only as secretion of the bee. However, other sources of amylase production, such as microbes in the guts of bees remained to be studied. In our research, we compared the differences in the amylase values in rape flower nectar and honey in the stomachs of bees. Then, culture-dependent and culture-independent (16sDNA) methods were used to isolate and identify bacteria in rape flower nectar and bee stomach honey. The dominant bacteria were added to nectar, and the amylase...

Evaluation of pyriproxyfen, a juvenile hormone analog, on Drosophila melanogaster (Diptera: Drosophilidae): Insecticidal activity, ecdysteroid contents and cuticle formation


Eur. J. Entomol. 112 (4): 625-631, 2015 | DOI: 10.14411/eje.2015.084

The efficacy of pyriproxyfen, a juvenile hormone analog (JHA), was evaluated using third instar larvae of Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae). Various doses of the compound, ranging from 0.01 to 2 ng/larva, were applied topically to larvae (12 h before pupariation). Treatment did not prevent pupariation but inhibited adult emergence at all the doses tested. In a second series of experiments the ecdysteroid content of pupae was determined following application of pyriproxyfen at two doses, 0.108 and 0.29 ng/larva, corresponding to ID25 and ID50, the doses required for 25 and 50% inhibition of adult...

A comprehensive study of the changes in ecdysteroid levels during the feeding phase of fifth instar larvae of the silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Bela KESHAN, Bembem THOUNAOJAM, Sanathoibi D. KH

Eur. J. Entomol. 112 (4): 632-641, 2015 | DOI: 10.14411/eje.2015.088

The present study describes changes in haemolymph ecdysteroid levels during the feeding phase of fifth instar larvae of Bombyx mori. A small and sharp peak in ecdysteroid level was recorded on day 4 when larvae had already reached the critical weight (2.76 ± 0.11 g) for metamorphosis. Food deprivation of larvae from day 4 resulted in an increase in the ecdysteroid level. Injection of insulin in both 1 day (before reaching critical weight) and 4 day (after reaching critical weight) old fed larvae resulted in an increase in the ecdysteroid level. However, when deprived of food only the haemolymph ecdysteroid levels of 4 day old larvae respond...

Effect of photoperiod on adult size and weight in Harmonia axyridis (Coleoptera: Coccinellidae)


Eur. J. Entomol. 112 (4): 642-647, 2015 | DOI: 10.14411/eje.2015.081

Short photoperiods often accelerate preimaginal development in insects that overwinter as adults and thus ensure the timely development of the diapausing stage. However, fast development usually results in small adults with few reserves whereas successful overwintering is dependent on sufficient reserves of nutrients. Thus, an insect faces two opposite challenges: increase adult weight or speed up pre-adult development. One solution to this "time-size trade-off" is fast development resulting in relatively light-weight but not too small adults, which are able to survive if there is an early onset to winter and have the possibility of further increasing...

Age dependent mate choice influences reproductive and progeny attributes in aphidophagous ladybird beetles (Coleoptera: Coccinellidae)


Eur. J. Entomol. 112 (4): 648-657, 2015 | DOI: 10.14411/eje.2015.082

Despite the many studies on age based mate choice in insects, the question of how age influences mate choice in predaceous ladybird beetles is still unexplored. The present study evaluates age based mate choice in two sympatric aphidophagous ladybirds, Coccinella septempunctata L. and Coccinella transversalis Fab., in terms of mating behaviour (latent period, number of bouts, bout interval and duration of wriggling movements and mating), fecundity, egg viability and the development and survival of their progeny. Results revealed that middle aged (30-40 days) mates were preferred by young (10-15 days post-eclosion), middle aged (30-40...

Tuta absoluta (Lepidoptera: Gelechiidae): Thermal requirements and effect of temperature on development, survival, reproduction and longevity

Flavia da Silva KRECHEMER, Luis Amilton FOERSTER

Eur. J. Entomol. 112 (4): 658-663, 2015 | DOI: 10.14411/eje.2015.103

Tuta absoluta (Meyrick, 1917) is the main pest of tomato crops in South America. Recently, it was also reported in several countries in Europe and the Middle East. Because of its invasive potential and the lack of information on the biology of T. absoluta, the present study aimed to estimate its thermal requirements and evaluate the effect of temperature on its development, survival, reproduction and longevity. The experiments were carried out at constant temperatures of 10, 15, 20, 25 and 30°C. T. absoluta completed its development at all these temperatures. The thermal requirements were estimated by linear regression; the insects...

Effect of temperature on the survival and development of the immature stages of Monosteira unicostata (Hemiptera: Tingidae)


Eur. J. Entomol. 112 (4): 664-675, 2015 | DOI: 10.14411/eje.2015.087

The poplar lace bug, Monosteira unicostata (Mulsant & Rey), is one of the most important pests of almond trees in the Mediterranean area. The developmental times and survival of the immature stages of this tingid were determined at 16, 19, 22, 25, 28, 31, 34, 35.5, 37 and 39°C, 60 ± 10% relative humidity and under a 16L : 8D photoperiod. At 16 and 39°C, 100% mortality was recorded during immature development, with the egg, first and second nymphal instars the most susceptible. The lowest mortality was recorded at 28°C (9.8%). As temperature increased, the total developmental period decreased and the shortest duration was recorded at...

Analysis of transcripts of heat shock protein genes in silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Nalavadi CHANDRAKANTH, Kangayam M. PONNUVEL, Shunmugam M. MOORTHY, Sirigineedi SASIBHUSHAN, Vankadara SIVAPRASAD

Eur. J. Entomol. 112 (4): 676-687, 2015 | DOI: 10.14411/eje.2015.094

Silkworm is a poikilothermic insect, whose growth and development is significantly affected by high temperatures. The current study aimed to identify bivoltine breeds tolerant of the high temperature conditions that occur in the tropics. Percentage pupation at high temperatures and heat shock responses of silkworms were used as measures of thermotolerance. Thermotolerance of 20 silkworm breeds was assessed by rearing them at 36°C. Based on percentage pupation, three breeds, namely Nistari (multivoltine), SK4C (bivoltine) and CSR2 (bivoltine) were designated tolerant, moderately tolerant and susceptible, respectively. To understand the heat shock responses...

Organization of honeydew collection by foragers of different species of ants (Hymenoptera: Formicidae): Effect of colony size and species specificity


Eur. J. Entomol. 112 (4): 688-697, 2015 | DOI: 10.14411/eje.2015.077

Aphid honeydew is one of the main energy sources for various ants in the temperate zone, nevertheless relatively little is known about the organization of the work of honeydew foragers (aphid milkers). This study focuses on the honeydew collecting strategies used by different ants in steppe and forest multi-species communities in Western Siberia. The behaviour of marked foragers of 12 species (Formica - 7, Lasius - 2, Camponotus - 1, Myrmica - 2) was recorded. Depending on the degree of the aphid milker specialization and degree of protection of the aphids five honeydew collecting strategies of various complexity were distinguished:...

Invasive chestnut gall wasp Dryocosmus kuriphilus (Hymenoptera: Cynipidae), its native parasitoid community and association with oak gall wasps in Slovenia

Katarina KOS, Eva KRISTON, George MELIKA

Eur. J. Entomol. 112 (4): 698-704, 2015 | DOI: 10.14411/eje.2015.091

The Asian chestnut gall wasp (ACGW), Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), is a global pest of chestnut trees. This pest was first recorded in Slovenia in 2005. Despite strict phytosanitary measures it was present throughout the country in native chestnut stands by 2013. We provide here the first overview of the parasitoids of ACGW in Slovenia recorded over a 4-year period and the direct interactions between parasitoid communities attacking hosts on chestnut and oaks at the same sites. A total of 27 species of native parasitoids that normally parasitize oak cynipids emerged from ACGW galls. The most abundant species were...

Invasive ant Tapinoma melanocephalum (Hymenoptera: Formicidae): A rare guest or increasingly common indoor pest in Europe?


Eur. J. Entomol. 112 (4): 705-712, 2015 | DOI: 10.14411/eje.2015.089

The ghost ant (GA), Tapinoma melanocephalum (Fabricius, 1793), is one of the most common invasive species of ants (Hymenoptera: Formicidae) in the world. While in its native tropical region it is both an ordinary indoor and outdoor pest, it occurs only indoors in the temperate zone, where increasingly more records of this species have been published since 1887. In this study, the current distribution of GA in Europe, including the new records published since Wetterer (2009), is reviewed. Furthermore, we report the first record of GA in the Czech Republic, where a large colony of GA was discovered in a block of flats in České Budějovice in 2014,...

Bactrocera oleae (Diptera: Tephritidae) in Iran: An invasion from the Middle West


Eur. J. Entomol. 112 (4): 713-721, 2015 | DOI: 10.14411/eje.2015.097

Despite an age-old tradition of olive growing and its geographical location, Iran was apparently free of the olive fly, Bactrocera oleae (Rossi, 1790) (Diptera: Tephritidae), the major worldwide olive tree pest, until the last decade. However, this situation has changed radically since B. oleae was first reported in 2004, and the olive fly is now Iran's most important pest of olive trees, and a very serious threat to olive production in the Northern and more humid parts of the country. Genetic analyses of mitochondrial markers of B. oleae collected in the traditional olive growing area in Northwestern Iran were used to determine...

Sociogenetic structure in nests of the mud dauber wasp Trypoxylon (Trypargilum) albitarse (Hymenoptera: Crabronidae)


Eur. J. Entomol. 112 (4): 722-727, 2015 | DOI: 10.14411/eje.2015.092

Trypargilum is a subgenus of solitary spider-hunting wasps whose males guard the nest, an unusual behaviour for male wasps. A male pairs with a female and copulates repeatedly with her during the nesting process, although females regularly copulate with satellite males, which employ an alternative reproductive strategy. The purpose of this paper was to determine the sociogenetic structure in twenty-nine nests of Trypoxylon albitarse sampled at six sites in Brazil. A total of 367 wasps were genotyped for eight species-specific polymorphic microsatellite loci. Genotypic segregation analyses were conducted to test whether the nests sampled...

Changes in ladybird (Coleoptera: Coccinellidae) communities along a steep pollution gradient in subarctic forests of European Russia

Mikhail V. KOZLOV

Eur. J. Entomol. 112 (4): 728-733, 2015 | DOI: 10.14411/eje.2015.075

Industrial air pollution constitutes a major environmental disturbance, and its impacts on insect communities have considerable implications for ecosystem structure and functions. Existing information on insect responses to pollution generally addresses abundant taxa, whereas few studies examine the effects of pollution on rare species. To begin rectifying this bias, ladybirds (Coleoptera: Coccinellidae) were quantitatively collected annually from 2002-2014 in 13 sites located 0.7 to 39.7 km from the nickel-copper smelter in Monchegorsk, north-western Russia. Seven of eight recorded species were found only in severely and /or moderately polluted sites....

Responses of grassland arthropods to various biodiversity-friendly management practices: Is there a compromise?

Monika MAZALOVÁ, Jan ŠIPOŠ, Stanislav RADA, Josef KAŠÁK, Bořivoj ŠARAPATKA, Tomáš KURAS

Eur. J. Entomol. 112 (4): 734-746, 2015 | DOI: 10.14411/eje.2015.076

The rich species pool in semi-natural grasslands is associated with high spatial and temporal heterogeneity. This empirical study is one of the first to jointly analyze the response of orders, individual species and functional richness of arthropods to particular management practices and to linear landscape features, such as strips of grass and belts of trees. Mostly it was the less mobile, flightless taxa that were negatively affected by immediate disturbance caused by mowing. At a larger time-scale, grasslands managed by mowing once every year, and especially by a combined regime of mowing and grazing, supported the highest species richness of butterflies...

Is the type of soil an important factor determining the local abundance of carrion beetles (Coleoptera: Silphidae)?


Eur. J. Entomol. 112 (4): 747-754, 2015 | DOI: 10.14411/eje.2015.071

Carrion beetles (Coleoptera: Silphidae) provide a valuable ecosystem service by promoting nutrient cycling and controlling pests like noxious flies (Diptera: Calliphoridae and Sarcophagidae). Our main goal was to examine the relationship between the occurrence of carrion beetles and soil type. We used pitfall traps to collect 43,856 specimens of 15 species of carrion beetles in the Czech Republic during 2009. We found that the abundance of seven of the carrion beetles - Nicrophorus antennatus (Reitter), N. germanicus (Linnaeus), N. humator (Gleditsch), N. interruptus (Stephens), N. sepultor (Charpentier),...

Hoverfly (Diptera: Syrphidae) richness and abundance vary with forest stand heterogeneity: Preliminary evidence from a montane beech fir forest


Eur. J. Entomol. 112 (4): 755-769, 2015 | DOI: 10.14411/eje.2015.083

Hoverflies (Diptera: Syrphidae) provide crucial ecological services and are increasingly used as bioindicators in environmental assessment studies. Information is available for a wide range of life history traits at the species level for most Syrphidae but little is recorded about the environmental requirements of forest hoverflies at the stand scale. The aim of this study was to explore whether the structural heterogeneity of a stand influences species richness or abundance of hoverflies in a montane beech-fir forest. We used the catches of Malaise traps set in 2004 and 2007 in three stands in the French Pyrenees, selected to represent a wide range...

Effect of military activity on butterfly (Lepidoptera) communities in Korea: Conservation and maintenance of red listed species

Sung-Soo KIM, Tae-Sung KWON, Cheol Lee MIN

Eur. J. Entomol. 112 (4): 770-777, 2015 | DOI: 10.14411/eje.2015.099

Military training areas are increasingly recognized as areas of high biodiversity and habitats for many wild organisms, including threatened or endangered species. However, the information on the ecological value of military training areas is limited because it is difficult access these sites. The aim of this study is to evaluate the effect of military activity on butterfly communities. The survey was carried out in a military training area (MTA) at Inje-gun near the demilitarized zone (DMZ), Inje forest (IJF) a secondary forest and Gwangneung forest (GWF) an old growth forest, from April to October 2008 to 2011. IJF and GWF were selected in order...

Cascading ecological effects caused by the establishment of the emerald ash borer Agrilus planipennis (Coleoptera: Buprestidae) in European Russia


Eur. J. Entomol. 112 (4): 778-789, 2015 | DOI: 10.14411/eje.2015.102

Emerald ash borer, Agrilus planipennis, is a destructive invasive forest pest in North America and European Russia. This pest species is rapidly spreading in European Russia and is likely to arrive in other countries soon. The aim is to analyze the ecological consequences of the establishment of this pest in European Russia and investigate (1) what other xylophagous beetles develop on trees affected by A. planipennis, (2) how common is the parasitoid of the emerald ash borer Spathius polonicus (Hymenoptera: Braconidae: Doryctinae) and what is the level of parasitism by this species, and (3) how susceptible is the native European...

Sibling species delimitation and nomenclature of the Merodon avidus complex (Diptera: Syrphidae)

Dunja POPOVIĆ, Jelena AČANSKI, Mihajla DJAN, Dragana OBREHT, Ante VUJIĆ, Snežana RADENKOVIĆ

Eur. J. Entomol. 112 (4): 790-809, 2015 | DOI: 10.14411/eje.2015.100

A study of the relationships between 21 southern European, Moroccan and Turkish populations of the Merodon avidus species complex was carried out. Based on a parallel study of type material from several museums, documented diagnostic morphological characters, season of adult activity and geographical distribution, we justify the use of the following names for three closely related taxa in this complex: M. avidus (Rossi, 1790), M. moenium (Wiedemann, 1822), and M. ibericus Vujić nom. n. (new name for M. bicolor Gil Collado, 1930, preoccupied by M. bicolor Walker, 1852). Neotypes of Merodon avidus, M....

Wing morphology is linked to stable isotope composition of nitrogen and carbon in ground beetles (Coleoptera: Carabidae)

Marcin ZALEWSKI, Dorota DUDEK-GODEAU, Alexei V. TIUNOV, Jean-François GODEAU, Yutaka OKUZAKI, Hiroshi IKEDA, Paweł SIENKIEWICZ, Werner ULRICH

Eur. J. Entomol. 112 (4): 810-817, 2015 | DOI: 10.14411/eje.2015.072

As movement is expensive in terms of energy required, mobile animals might have to utilize more energy rich resources than less mobile animals. As mobile animals are possibly more effective foragers we hypothesize a positive correlation between mobility and trophic niche width. We address this link using information on the trophic level of 35 winged, wingless and wing dimorphic species of ground beetles studied on 18 lake islands and at two mainland sites in northern Poland. Trophic analyses using stable isotope values (δ15 N, δ13C) revealed that winged individuals of wing dimorphic species are characterized by broader...

Natural breakage of the very long intromittent organ of the seed bug Lygaeus simulans (Heteroptera: Lygaeidae)


Eur. J. Entomol. 112 (4): 818-823, 2015 | DOI: 10.14411/eje.2015.098

The male intromittent organ of the seed bug Lygaeus simulans ends in a long, sclerotized structure which is used to transfer sperm during mating. Observations suggest that this structure becomes brittle and is liable to breakage after being artificially exposed to the air for an extended period of time. In this study we investigate the frequency of intromittent organ breakage in L. simulans. We first examined the intromittent organ of a sample of males that mated once, and found that breakage was rare. We hypothesised that breakages are likely to be more frequent if a male is able to mate multiple times, and so we next paired males with...

Epitrix papa sp. n. (Coleoptera: Chrysomelidae: Galerucinae: Alticini), previously misidentified as Epitrix similaris, is a threat to potato production in Europe


Eur. J. Entomol. 112 (4): 824-830, 2015 | DOI: 10.14411/eje.2015.096

A nonnative pest of potato recently established and causing significant economic damage in Portugal and Spain was initially identified as Epitrix similaris Gentner and included on lists of quarantine pests. The identity of this pest was doubted by some experts, since E. similaris is a rare species previously recorded only from California and does not damage potato in its native range. Our comparison of 20 specimens of this pest from Portugal with paratypes of E. similaris has revealed that it is not E. similaris. The name "Epitrix similaris" should be removed from lists of quarantine pests. The damage to potato tubers...

Phylogenetic relationships and larval morphology of the recently described diving beetle genus Laccomimus (Coleoptera: Dytiscidae: Laccophilinae)

Mariano C. MICHAT, Mario TOLEDO

Eur. J. Entomol. 112 (4): 831-843, 2015 | DOI: 10.14411/eje.2015.085

The larvae of the diving beetle genus Laccomimus Toledo & Michat, 2015 (Laccophilinae) are studied for the first time, based on detailed descriptions and illustrations of all instars of L. distinctus Toledo & Michat, 2015, with particular emphasis on morphometry and chaetotaxy. The phylogenetic relationships of this genus within the tribe Laccophilini are analyzed cladistically. Laccomimus is recovered as part of a clade that includes the genera Australphilus Watts, 1978, Neptosternus Sharp, 1882 and Laccophilus Leach, 1815 and is characterized by the presence of natatory setae on the tibia and tarsus,...

Partially disarticulated new Miocene burrower bug (Hemiptera: Heteroptera: Cydnidae) from Cerová (Slovakia) documents occasional preservation of terrestrial arthropods in deep-marine sediments

Peter VRŠANSKÝ, Jerzy A. LIS, Ján SCHLÖGL, Martin GULDAN, Tomáš MLYNSKÝ, Peter BARNA, Pavel ŠTYS

Eur. J. Entomol. 112 (4): 844-854, 2015 | DOI: 10.14411/eje.2015.090

The state of preservation of the bug Sehirus carpathiensis J.A. Lis, Vršanský & Schlögl, sp. n. (Hemiptera: Heteroptera: Cydnidae) from the Lakšárska Nová Ves Formation at Cerová (Slovakia) supports extremely rapid sinking and burial in upper bathyal Early Miocene sediments. The specimen originated from land at most a few kilometers away, but transport via a river can be excluded as the head and wings are still attached. Its most likely source based on fossil flora and the habitat of several living representatives of the genus is a riparian habitat in an adjacent area of land. Phylogenetically the new species is closely related to both Oligocene...

New set of microsatellite markers for the spotted-wing Drosophila suzukii (Diptera: Drosophilidae): A promising molecular tool for inferring the invasion history of this major insect pest

Antoine FRAIMOUT, Anne LOISEAU, Donald K. PRICE, Anne XUÉREB, Jean-François MARTIN, Renaud VITALIS, Simon FELLOUS, Vincent DEBAT, Arnaud ESTOUP

Eur. J. Entomol. 112 (4): 855-859, 2015 | DOI: 10.14411/eje.2015.079

Historical and observational data for invasive species are often sparse and incomplete, so molecular genetic markers are increasingly used and have proved to be efficient tools to make inferences about invasion histories. Here, we report the development of 28 polymorphic microsatellite markers in the invasive spotted-wing drosophila, Drosophila suzukii (Matsumura, 1931) (Diptera: Drosophilidae) designed from recent genomics resources, and their cross-amplification in closely related Drosophila species of the suzukii subgroup. The markers, located on autosomal chromosomes, were characterized in two distinct invasive populations...

Modification of the rotating model method for studying the sexual discriminative abilities of butterflies (Lepidoptera)


Eur. J. Entomol. 112 (4): 860-861, 2015 | DOI: 10.14411/eje.2015.078

Recently, Imafuku & Kitamura (Eur. J. Entomol. 112, 2015: 328-333) succeeded in inducing males of two territorial butterflies, Chrysozephyrus smaragdinus and Neozephyrus japonicus, to respond to wing models by rotating the models. Based on the results of two-choice tests in which males stayed longer within 50 mm of the female model than the male model the authors claim that males of both of these butterflies identify females using the patterns on the wings. I think this study provides a new method for studying the discriminative abilities of butterflies. In contrast to static models, however, butterflies attracted to...

Prey abundance and intraguild predation between Adalia bipunctata (Coleoptera: Coccinellidae) and Macrolophus pygmaeus (Hemiptera: Miridae)

Vincenzo TROTTA, Juliana DURÁN PRIETO, Paolo FANTI, Donatella BATTAGLIA

Eur. J. Entomol. 112 (4): 862-865, 2015 | DOI: 10.14411/eje.2015.080

Macrolophus pygmaeus Rambur (Hemiptera: Miridae) and Adalia bipunctata (L.) (Coleoptera: Coccinellidae) are two predatory insects commonly used as biological control agents. In order to determine the incidence with which both species attack and eat each other [Intraguild predation (IGP)], the direction and symmetry of the interaction between A. bipunctata and M. pygmaeus were characterized. In addition, whether the intensity of IGP between these two predators increased when the number of extraguild prey Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae) decreased, was also determined. Unidirectional sensu stricto...