Eur. J. Entomol. 119: 232-241, 2022 | DOI: 10.14411/eje.2022.025

Effect of solvent extraction time on the hydrocarbon profile of Drosophila suzukii (Diptera: Drosophilidae) and behavioural effects of 9-pentacosene and dodecaneOriginal article

Maria BELENIOTI ORCID...1, Emmanouil RODITAKIS ORCID...2, Manolis SOFIADIS ORCID...3, Maria FOUSKAKI ORCID...1, Maria APOSTOLAKI ORCID...4, Nikos CHANIOTAKIS ORCID...1
1 Laboratory of Analytical Chemistry, Department of Chemistry, University of Crete, Panepistimioupoli Vouton, 70013 Heraklion, Crete, Greece; e-mails: maria.belenioti@uoc.gr, fouskakm@uoc.gr, chaniotakis@uoc.gr
2 Department of Agriculture, Hellenic Mediterranean University, Stavromenos PC 71410, Heraklion, Crete, Greece; e-mail: eroditakis@hmu.gr
3 Laboratory of Organic Chemistry, Department of Chemistry, University of Crete, Panepistimioupoli Vouton, 70013 Heraklion, Crete, Greece; e-mail: msofiadis@gmail.com
4 Laboratory of Environmental Chemistry, Department of Chemistry, University of Crete, Panepistimioupoli Vouton, 70013 Heraklion, Crete, Greece; e-mail: apostolm@uoc.gr

Hydrocarbons play a major role in the life cycle of insects. Their composition and concentration can be affected by several factors. Hydrocarbons are biosynthesized in oenocytes and subsequently transported to the cuticle of insects, such as Drosophila suzukii (Matsumura) (Diptera: Drosophilidae). As the extraction procedure markedly affects the type and amount of hydrocarbon obtained we determined the association between the time taken to extract the maximum amounts of these compounds and the behaviour of D. suzukii. The required extraction time to reach a steady state is different for each hydrocarbon, which in most cases is more than one hour. On the other hand, if the entire hydrocarbon profile of D. suzukii needs to be investigated, extraction times significantly longer than one hour were required. By extending the extraction time 5 additional hydrocarbons were detected in D. suzukii for the first time. One of them, dodecane proved to be repulsive to D. suzukii. In addition, it took 3 h of extraction to determine the maximum value of 9-pentacosene, which is responsible for triggering mating behaviour in D. suzukii.

Keywords: Cuticle hydrocarbons, bioassays, extraction steady state

Received: August 31, 2021; Revised: June 24, 2022; Accepted: June 24, 2022; Published online: July 26, 2022  Show citation

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BELENIOTI, M., RODITAKIS, E., SOFIADIS, M., FOUSKAKI, M., APOSTOLAKI, M., & CHANIOTAKIS, N. (2022). Effect of solvent extraction time on the hydrocarbon profile of Drosophila suzukii (Diptera: Drosophilidae) and behavioural effects of 9-pentacosene and dodecane. EJE, 119, Article 232-241. https://doi.org/10.14411/eje.2022.025
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