Eur. J. Entomol. 103 (2): 459-467, 2006 | DOI: 10.14411/eje.2006.059

Phylogeny of Chrysotoxum species (Diptera: Syrphidae) inferred from morphological and molecular characters

Antonio MASETTI1, Andrea LUCHETTI2, Daniele SOMMAGGIO3, Giovanni BURGIO1, Barbara MANTOVANI*,2
1 Dipartimento di Scienze e Tecnologie Agroambientali - Entomologia, Alma Mater Studiorum - UniversitL di Bologna, viale G. Fanin 42, 40127 Bologna, Italy
2 Dipartimento di Biologia Evoluzionistica Sperimentale, Alma Mater Studiorum - UniversitL di Bologna, via Selmi 3, 40126 Bologna, Italy
3 Biostudio, via Riello 4, 36010 Velo d'Astico, Vicenza, Italy

Relationships of nine Italian Chrysotoxum species were analysed using morphological and molecular data. The morphology-derived cladogram revealed three well-defined groups: (i) C. cautum, (ii) the arcuatum group (C. arcuatum, C. fasciolatum) and (iii) the festivum group (C. festivum - C. vernale, C. bicinctum, C. elegans, C. octomaculatum and C. parmense). Trees inferred from COI-tRNALeu-COII sequences were largely in agreement, but they identified (i) C. parmense as an isolated branch, (ii) C. festivum and C. vernale as separate entities, (iii) C. elegans within a paraphyletic C. festivum clade. ITS2 trees were partially unresolved but C. parmense sequence emerged as a sister to the festivum group. The monophyly of the festivum group derived from morphological data was rejected by a phylogenetic test performed on combined molecular data set. The diagnostic value of some morphological characters commonly used to identify Chrysotoxum species is therefore questioned.

Keywords: Chrysotoxum, cytochrome oxidase I, cytochrome oxidase II, molecular phylogeny, morphology, tRNALeu

Received: May 31, 2005; Revised: September 9, 2005; Accepted: September 9, 2005; Published: April 6, 2006  Show citation

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MASETTI, A., LUCHETTI, A., SOMMAGGIO, D., BURGIO, G., & MANTOVANI, B. (2006). Phylogeny of Chrysotoxum species (Diptera: Syrphidae) inferred from morphological and molecular characters. EJE, 103(2), 459-467. doi: 10.14411/eje.2006.059
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    References

    1. BLAXTER M. 2004: The promise of a DNA taxonomy. Phil. Trans. R. Soc. London. (B) 359: 669-679 Go to original source...
    2. BRADESCU V. 1991: Les Syrphides de Roumanie (Diptera, Syrphidae), cles de determination et repartition. Trav. Mus. Hist. Nat. Grigore Antipa 31: 7-83
    3. CATERINO M.S., CHO S. & SPERLING F.A.H. 2000: The current state of insect molecular systematics: a thriving tower of Babel. Annu. Rev. Entomol. 45: 1-54 Go to original source...
    4. CHENG X., LU J., HUANG C., DAI Z. & ZHANG G. 2000: Determination of phylogenetic position of Pipizini (Diptera, Syrphidae): based on molecular, biological and morphological data. Sci. China (C) 42: 146-156 Go to original source...
    5. DACCORDI M. & SOMMAGGIO D. 2002: Syrphidae. Boll. Soc. Entomol. It. 134: 192-198
    6. DEAN M.D. & BALLARD W.O. 2001: Factors affecting mitochondrial DNA quality from museum preserved Drosophila simulans. Entomol. Exp. Appl. 98: 279-283. Go to original source...
    7. GIGLIO TOS E. 1890: Le specie europee del genere Chrysotoxum Meig. Atti Soc. Sci. Torino 26: 134-165
    8. GOELDLIN DE TIEFENAU P. 1976: Revision du genre Paragus (Dipt. Syrphidae) de la region palearctique occidentale. Bull. Soc. Entomol. Suisse 49: 79-108
    9. HERBERT P.D., RATNASINGHAM S. & DEWAARD J. 2003: Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc. R. Soc. London. (B) (Suppl.) 270: S96-S99 Go to original source...
    10. ICZN 2001: Opinion 1982 (Case 3090): Musca arcuata Linnaeus, 1758 and M. festiva Linnaeus, 1758 (currently Chrysotoxum arcuatum and C. festivum) and M. citrofasciata De Geer, 1776 (currently Xanthogramma citrofasciatum) (Insecta, Diptera): specific names conserved by the designation of neotypes for M. arcuata and M. festiva. Bull. Zool. Nom. 58: 241-242
    11. KUMAR S., TAMURA K. & NEI M. 2004: MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. 5: 150-163 Go to original source...
    12. LOEW H. 1841: Uber die gattung Chrysotoxum. Stettin. Entomol. Ztg 2: 136-141, 155-160
    13. MANONMANI A., TOWNSON H., ADENIRAN T., JAMBULINGAM P., SAHU S. & VIJAYAKUMAR T. 2001: rDNA-ITS2 polymerase chain reaction assay for the sibling species of Anopheles fluviatilis. Acta Trop. 78: 3-9 Go to original source...
    14. MEIGEN J.W. 1800: Nouvelle classification des mouches L deux ailes (Diptera L.), d'apres un plan tout nouveau. Perronneau, Paris, 40 pp Go to original source...
    15. MILANKOV V., STAMENKOVIC J., LUDOSKI J., STAHLS G. & VUJIC A. 2005: Diagnostic molecular markers and genetic relationships among three species from the Cheilosia canicularis group (Diptera: Syrphidae). Eur. J. Entomol. 102: 125-131 Go to original source...
    16. POSADA D. & CRANDALL K. 1998: MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817-818 Go to original source...
    17. PECK L.V. 1988: Family Syrphidae. In Soos A. & Papp L. (eds): Catalogue of Palearctic Diptera. Syrphidae - Conopidae. Elesevier, Amsetrdam, pp. 11-230
    18. RONDANI C. 1845: Species Italicae generis Chrysotoxi insectis Dipteris, observatae et distinctae. Fragmentum decimum ad inserviendum dipterologiae Italicae. Ann. Soc. Entomol. Fr. 3: 193-203
    19. ROTHERAY G. & GILBERT F. 1999: Phylogeny of palaearctic Syrphidae (Diptera): evidence from larval stages. Zool. J. Linn. Soc. 127: 1-112 Go to original source...
    20. SAMBROOK J., FRITSCH E.T. & MANIATIS T. 1989: Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
    21. SEGUY E. 1961: Dipteres Syrphides de l'Europe occidentale. Mem. Mus. Nat. Hist. Nat. (N.S.) (A, Zool.) 23: 1-248
    22. SHANNON R.C. 1926: The Chrysotoxine Syrphid-flies. Proc. U.S. Natl Mus. 69: 1-20 Go to original source...
    23. SHIMODAIRA H. & HASEGAWA M. 1999: Multiple comparison of log-likelihood with applications to phylogenetic inference. Mol. Biol. Evol. 16: 1114-1116 Go to original source...
    24. SIMON C., FRATI F., BECKENBACH A., CRESPI B., LIU H. & FLOOCK P. 1994: Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am. 87: 651-701 Go to original source...
    25. SKEVINGTON J.H. & YEATES D.K. 2000: Phylogeny of the Syrphoidea (Diptera) inferred from mtDNA sequences and morphology with particular reference to classification of the Pipunculidae (Diptera). Mol. Phylogenet. Evol. 16: 212-224 Go to original source...
    26. SOMMAGGIO D. 2001: The species of the genus Chrysotoxum Meigen, 1822 (Diptera, Syrphidae) described by Giglio Tos. Boll. Mus. Reg. Sci. Nat. Torino 18: 115-126
    27. SPEIGHT M.C.D. 2004: Species account of European Syrphidae (Diptera) 2004. In Speight M.C.D., Castella E., Sarthou J.-P. & Monteil C. (eds): Syrph the Net: The Database of European Syrphidae. Syrph the Net publications, Dublin, 237 pp
    28. STAHLS G. & NYBLOM K. 2000: Phylogenetic analysis of the genus Cheilosia (Diptera, Syrphidae) using mitochondrial COI sequence data. Mol. Phylog. Evol. 15: 235-241 Go to original source...
    29. STAHLS G., HIPPA H., ROTHERAY G., MUONA J. & GILBERT F. 2003: Phylogeny of Syrphidae (Diptera) inferred from combined analysis of molecular and morphological characters. Syst. Entomol. 28: 433-450 Go to original source...
    30. STUBBS A.E. & FALK S.J. 1983: British Hoverflies, an Illustrated Identification Guide. British Entomological and Natural History Society, London, 253 pp
    31. SWOFFORD D.L. 2001: PAUP* - Phylogenetic Analysis Using Parsimony (*and Other Methods). Sinauer Associated, Sunderland, MA
    32. THOMPSON F.C., VOCKEROTH J.R. & SPEIGHT M.C.D. 1982: The Linnaean species of flower flies (Diptera: Syrphidae). Mem. Entomol. Soc. Wash. 10: 150-165
    33. VERLINDEN L. 1999: A new Pipizella (Diptera, Syrphidae) from the French and Italian Alps, with a key to the Pipizella species of Central and Western Europe. Volucella 4: 11-27
    34. VIOLOVITCH N.A. 1974: A review of the palearctic species of the genus Chrysotoxum Mg. (Diptera, Syrphidae). Entomol. Obozr. 53: 196-217
    35. VOCKEROTH J.R. 1992: The flower flies of the subfamily Syrphinae of Canada, Alaska and Greenland. In The Insects and Arachnids of Canada. Part 18. Agriculture Canada, Ottawa, 456 pp
    36. WINNEPENNINCKX B., BACKELJAU T. & WACHTER R. 1993: Extraction of high molecular weight DNA from molluscs. Trends Genet. 9: 407 Go to original source...
    37. YOUNG I. & COLEMAN A.W. 2004: The advantages of the ITS2 region of the nuclear rDNA cistron for analysis of phylogenetic relationships of insects: a Drosophila example. Mol. Phylog. Evol. 30: 236-242 Go to original source...
    38. ZUCKER M. 2003: Mfold web server for nucleic acid folding and hybridization prediction. Nuc. Ac. Res. 31: 3406-3415 Go to original source...

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