Eur. J. Entomol. 104 (1): 67-72, 2007 | DOI: 10.14411/eje.2007.011

Development of the entomopathogenic hyphomycete Lecanicillium muscarium (Hyphomycetes: Moniliales) on various hosts

Hassan ASKARY, Hamid YARMAND
Plant Protection Department, Research Institute of Forests and Rangelands, P.O. Box: 13185-116, Tehran, Iran;

The present scanning electron microscopy study describes the development of Lecanicillium muscarium, strain DAOM 198499, on the surface of diverse hosts, including Sphaerotheca fuliginea, a fungal host, and Macrosiphum euphorbiae and Aphidius nigripes, insect hosts. The hosts were sprayed with a conidial suspension of L. muscarium (107 conidia/ml). The specimens used in the SEM investigation were collected at particular periods after spraying and prepared for scanning using standard methods. Germination tubes developed twenty-four hours after applying L. muscarium conidia to each host. Hyphae were attached to the host by a thin mucilaginous matrix. Seventy-two hours after spraying, hyphae of S. fuliginea had collapsed and were encircled by the parasite, and primary sporulation of L. muscarium was observed. On the aphid host, colonization started with adherence of the conidia to the host cuticle, followed by conidial germination and growth of mycelium on the surface of the insect's integument. After 48 to 72 h, post colonization, the first sporulation was observed on the cuticle, particularly at articulations. The mode of parasitism of A. nigripes by this fungus was similar to that of the aphid. Development of L. muscarium was observed on both mummified aphids (containing the pupae of parasitoids) and adult parasitoids.

Keywords: Sphaerotheca fuliginea, Macrosiphum euphorbiae, Aphidius nigripes, Lecanicillium muscarium, host colonization, scanning electron microscopy

Received: April 20, 2006; Accepted: September 6, 2006; Revised: September 6, 2006; Published: January 3, 2007Show citation

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ASKARY, H., & YARMAND, H. (2007). Development of the entomopathogenic hyphomycete Lecanicillium muscarium (Hyphomycetes: Moniliales) on various hosts. Eur. J. Entomol.104(1), 67-72. doi: 10.14411/eje.2007.011.
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References

  1. ALLEN D.J. 1982: Verticillium lecanii on the bean rust fungus, Uromyces appendiculatus. Trans. Br. Mycol. Soc. 79: 362-364 Go to original source...
  2. ASKARY H. & BRODEUR J. 1999: Susceptibility of larval stages of aphid parasitoid, Aphidius nigripes to the entomopathogenic fungus Verticillium lecanii. J. Invert. Pathol. 73: 129-132 Go to original source...
  3. ASKARY H., BENHAMOU N. & BRODEUR J. 1997: Ultrastructural and cytochemical investigations of the antagonistic effect of Verticillium lecanii on cucumber powdery mildew. Phytopathology 87: 359-368 Go to original source...
  4. ASKARY H., CARRIERE Y., BELANGE R.R. & BRODEUR J. 1998: Pathogenicity of the fungus Verticillium lecanii to aphids and powdery mildew. Biocontr. Sci. Technol. 8: 23-32 Go to original source...
  5. ASKARY H., BENHAMOU N. & BRODEUR J. 1999: Characterization of aphid invasion by the hyphomycete Verticillium lecanii. J. Invert. Pathol. 74: 1-13 Go to original source...
  6. BENHAMOU N. & BRODEUR J. 2000: Evidence for antibiosis and induced hosts defense reactions in the interaction between Verticillium lecanii and Penicillium digitatum, the causal agent of green mold. Phytopathology 90: 932-943 Go to original source...
  7. BENHAMOU N. & BRODEUR J. 2001: Pre-inoculation of Ri T-DNA transformed cucumber roots with the mycoparasite, Verticillium lecanii, induces host defense reactions against Pythium ultimum infection. Physiol. Molec. Plant Pathol. 58: 133-146 Go to original source...
  8. BREY P.T., LATGE J.P. & PREVOST M.C. 1986: Integumental penetration of the pea aphid, Acyrthosiphon pisum, by Conidiobolus obscurus (Entomophthoraceae). J. Invert. Pathol. 48: 34-41 Go to original source...
  9. BRODEUR J. & MCNEIL J.N. 1994: Seasonal ecology of Aphidius nigripes (Hymenoptera: Aphidiidae), a parasitoid of Macrosiphum euphorbiae (Homoptera: Aphididae). Environ. Entomol. 23: 292-298 Go to original source...
  10. BROOKS W.M. 1993: Host-parasitoid-pathogen interactions. In Beckage N.E., Thompson S.N. & Federici B.A. (eds): Parasites and Pathogens of Insects: Pathogens. Academic Press, San Diego, pp. 231-272
  11. BURGE M.N. 1988: The scope of fungi in biological control. In Burge M.N. (ed.): Fungi in Biological Control System. Manchester University Press, UK, pp. 1-18
  12. CHARNLEY A.K. 1989: Mechanisms of fungal pathogenesis in insects. In Whipps J.M. & Lumsden R.D. (eds): The Biotechnology of Fungi for Improving Plant Growth. Cambridge University, London, pp. 85-125
  13. CHARNLEY A.K. & ST. LEGER R.J. 1991: The role of cuticledegrading enzymes in fungal pathogenesis in insects. In Cole G.T. & Hoch H.C. (eds): The Fungal Spore and Disease Initiation in Plants and Animals. Plenum Press, New York, pp. 267-286
  14. CLARKSON J.M. & CHARNLEY A.K. 1996: New insights into the mechanisms of fungal pathogenesis in insects. Trends Microbiol. 4:197-203 Go to original source...
  15. FLEXNER J.L., LIGHTHART B. & CROFT B.A. 1986: The effects of microbial pesticides on non-target, beneficial arthropods. Agric. Ecosyst. Environ. 16: 203-254 Go to original source...
  16. HAJEK A.E. & EASTBURN C.C. 2003: Attachment and germination of Entomophaga maimaiga conidia on host and non-host larval cuticle. J. Invert. Pathol. 82: 12-22 Go to original source...
  17. HALL R.A. 1980: Laboratory infection of insects by Verticillium lecanii strains isolated from phytopathogenic fungi. Trans. Br. Mycol. Soc. 74: 445-446 Go to original source...
  18. HALL R.A. 1981: The fungus Verticillium lecanii as a microbial insecticide against aphids and scales. In Burges H.D. (ed.): Microbial Control of Pests and Plant Diseases. Academic Press, London, pp. 483-498
  19. HEALE J.B., ISAAC J.E. & CHANDLER D. 1988: Prospects for strain improvement in entomopathogenic fungi. Pestic. Sci. 26: 79-92 Go to original source...
  20. MEYER S.L.F., HUETTEL R.N. & SAYRE R.M. 1990: Isolation of fungi from Heterodera glycines and in vitro bioassays for their antagonism to eggs. J. Nematol. 22: 532-537
  21. MOINO JR. A., ALVES S.B., LOPES R.B., NEVES P.M.O.J., PEREIRA R.M. & VIEIRA S.A. 2002: External development of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in the subterranean termite Heterotermes tenuis. Scientia Agric. 59: 267-273 Go to original source...
  22. RAGHAVENDRA-RAO N.N. & PAVGI M.S. 1977: Two mycoparasites on powdery mildews. Sydowia 30: 145-147
  23. SCHREITER G., BUTT T.M., BECKETT A., VESTERGAARD S. & MORITZ G. 1994: Invasion and development of Verticillium lecanii in the western flower thrips, Frankliniella occidentalis. Mycol. Res. 98: 1025-1034 Go to original source...
  24. SITCH J.C. & JACKSON C.W. 1997: Pre-penetration events affecting host specificity of Verticillium lecanii. Mycol. Res. 101: 535-541 Go to original source...
  25. SMITH R.J., PEKRUL S. & GRULA E.A. 1981: Requirement for sequential enzymatic activities for peneteration of the integument of the corn earworm (Heliothis zea). J. Invert. Pathol. 38: 335-344 Go to original source...
  26. SPENCER D.M. & ATKEY P.T. 1981: Parasitic effects of Verticillium lecanii on two rust fungi. Trans. Br. Mycol. Soc. 77: 535-542 Go to original source...
  27. ST. LEGER R.S. 1993: Biology and mechanisms of insect-cuticle invasion by Deuteromycete fungal pathogens. In Beckage N.E., Thompson S.N. & Federici B.A. (eds): Parasites and Pathogens of Insects. Vol. 2: Pathogens. Academic Press, San Diego, pp. 211-229
  28. WRAIGHT S.P., BUTT T.M., GALAINI-WRAIGHT S., ALLEE L.L., SOPER R.S. & ROBERTS D.W. 1990: Germination and infection processes of the entomophthoralean fungus Erynia radicans on the potato leafhopper, Empoasca fabae. J. Invert. Pathol. 56: 157-174 Go to original source...
  29. YAN J., LI L.Z., EVANS H.C. & GODVIN G.R. 1996: Observations on the micromorphology of Verticillium using cryo-scanning electron microscopy. Acta Mycol. Sin. 15: 34-37
  30. ZARE R. & GAMS W. 2001: A revision of Verticillium section Prostrata. IV. The genera Lecanicillium and Simplicillium gen. nov. Nova Hedvigia 73: 1-50

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