Eur. J. Entomol. 123: 175-184, 2026 | DOI: 10.14411/eje.2026.018
Saproxylic beetle assemblages in floodplain forests of Kopački rit Nature Park (Croatia): A baseline for Natura 2000 monitoringOriginal article
- Department of Biology, Josip Juraj Strossmayer University of Osijek, Ulica cara Hadrijana 8/A, 31000 Osijek, Croatia; e-mails: msag@biologija.unios.hr, nturic@biologija.unios.hr, goran.vignjevic@biologija.unios.hr, ngraovac@biologija.unios.hr
Saproxylic beetles are a key component of forest biodiversity and important indicators of forest naturalness, dead-wood continuity and habitat quality. Floodplain forests with old oak stands are among the most species-rich forest ecosystems in Europe, yet data on saproxylic beetle assemblages from such habitats remain scarce in south-eastern Europe. In this study, saproxylic beetles were investigated in three floodplain forest communities within Kopački rit Nature Park (eastern Croatia) using flight-intercept, pitfall and aerial attractant traps during the 2014 vegetation season. A total of 64 saproxylic beetle species from 14 families were recorded. Species richness and abundance were highest in oak-hornbeam forests, intermediate in poplar forests and lowest in willow-dominated stands, which nevertheless supported structurally distinct assemblages. Diversity indices and ordination analyses revealed a strong gradient in community structure from oak-dominated to willow-dominated floodplain forests. Flight-intercept traps were the most efficient sampling method, whereas other trap types provided complementary information on habitat-specific taxa. Several species listed on the European Red List of Saproxylic Beetles were recorded, including the Natura 2000 species Lucanus cervus and Cucujus cinnaberinus. Of particular importance was the detection of Rhysodes sulcatus, which triggered targeted faunistic surveys that confirmed its presence at additional Croatian localities and led to its formal inclusion in the Croatian Natura 2000 species list. Although the data were collected in 2014, they provide a valuable baseline for assessing saproxylic beetle diversity in natural floodplain forests and for the development of national Natura 2000 monitoring programmes. The results highlight the exceptional conservation value of Kopački rit as one of the best-preserved floodplain forest systems in the Continental biogeographical region.
Keywords: Coleoptera, biodiversity, conservation, sampling methods, oak, threatened species, dead wood, eastern Croatia
Received: February 9, 2026; Revised: May 12, 2026; Accepted: May 12, 2026; Published online: May 29, 2026 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Alexander K.N.A. 2008: Tree biology and saproxylic Coleoptera: issues of definitions and conservation language. - Rev. Ecol. (Terre Vie) 10: 9-13.
Go to original source... - Ballerio A., Rey A., Uliana M., Rastelli M., Rastelli S., Romano M. & Colacurcio L. 2010: Coleotteri scarabeoidei d'Italia. Tarantola, Brescia. [DVD in Italian]
- Baraud J. 1992: Coléoptères Scarabaeoidea d'Europe. Faune de France et Régions Limitrophes 78. Fédération française des Sociétés de Sciences naturelles, Paris, et Société linnéenne, Lyon, 856 pp.
- Bell R.T. & Bell J.R. 1978: Rhysodini of the world. Part I. A new classification of the tribe, and a synopsis of Omoglymmius subgenus Nitiglymmius. - Quaest. Entomol. 14: 43-88.
- Bell R.T. & Bell J.R. 1979: Rhysodini of the world. Part II. Revisions of the smaller genera. - Quaest. Entomol. 15: 377-446.
- Bell R.T. & Bell J.R. 2002: Two new species of Rhysodini (Coleoptera: Carabidae) with revised keys to Yamatosa Bell & Bell and Omoglymmius (Pyxiglymmius) Bell & Bell. - Stuttg. Beitr. Naturk. - 636: 1-7.
- Bell R.T. & Bell J.R. 2009: Rhysodine beetles (Insecta: Coleoptera: Carabidae): New species, new data III. - Ann. Carnegie Mus. 78: 45-77.
Go to original source... - Bense U. 1995: Longhorn Beetles. Illustrated Key to the Cerambycidae and Vesperidae of Europe. Margraf, Weikersheim, 512 pp.
- Bergman K.-O., Jansson N., Claesson K., Palmer M.W. & Milberg P. 2012: How much and at what scale? Multiscale analyses as decision support for conservation of saproxylic oak beetles. - For. Ecol. Manag. 265: 133-141.
Go to original source... - Bick H. 1989: Ökologie: Grundlagen, terrestrische und aquatische Ökosysteme, angewandte Aspekte. Gustav Fischer, Stuttgart, New York, 327 pp.
- Bonacci O., Tadić Z., Moržan A. & Radeljak I. 2002: Nature Park Kopački Rit Management Plan. Sector Study: Hydrology and Meteorology. Elektroprojekt, Zagreb, 177 pp.
- Bonacci T., Mazzei A., Horák J. & Brandmayr P. 2012: Cucujus tulliae sp. n. - an endemic Mediterranean saproxylic beetle from genus Cucujus Fabricius, 1775 (Coleoptera, Cucujidae), and keys for identification of adults and larvae native to Europe. - ZooKeys 212: 63-79.
Go to original source... - Bouget C., Brustel H., Brin A. & Noblecourt T. 2008: Sampling saproxylic beetles with window flight traps: Methodological insights. - Rev. Ecol. (Terre Vie) 10: 21-32.
Go to original source... - Buse J., Ranius T. & Assmann T. 2008: An endangered longhorn beetle associated with old oaks and its possible role as an ecosystem engineer. - Conserv. Biol. 22: 329-337.
Go to original source... - Cálix M., Alexander K.N.A., Nieto A., Dodelin B., Soldati F., Telnov D., Vazquez-Albalate X., Aleksandrowicz O., Audisio P., Istrate P. et al. 2018: European Red List of Saproxylic Beetles. IUCN, Brussels, 24 pp.
- Clarke K.R. & Warwick R.M. 2001: Change in Marine Communities: An Approach to Statistical Analysis and Interpretation. 2nd ed. PRIMER-E, Plymouth, 176 pp.
- Černi M. 2021: Faunal Research of Saproxylic Beetles in the Kopački Rit Nature Park. MSc thesis, Josip Juraj Strossmayer University of Osijek, 59 pp. [in Croatian, English abstract].
- Dražina T. & Temunović M. 2011: Research on Target Natura 2000 Beetle Species in Maksimir Park. Final report. BIOM Association, Zagreb, 24 pp. [in Croatian].
- Durbešić P., Vujčić-Karlo S. & Bukvić I. 1994: Faunistic and zoogeographic research of Coleoptera - Insecta in some forest communities on mountain Medvednica. - Šumarski list 118: 85-90.
- Đurić M. 2020: Inventory of Saproxylic Beetles in the Area of Biljski Rit and Zmajevački Dunavac. MSc thesis, Josip Juraj Strossmayer University of Osijek, 53 pp. [in Croatian, English abstract].
- Freude H., Harde K.W. & Lohse G.A. 1969: Die Käfer Mitteleuropas, Vol. 8. Goecke & Evers, Krefeld, 388 pp.
- Freude H., Harde K.W. & Lohse G.A. 1971: Die Käfer Mitteleuropas, Vol. 3. Goecke & Evers, Krefeld, 365 pp.
- Gossner M.M., Lachat T., Brunet J., Isacsson G., Bouget C., Brustel H., Brandl R., Weisser W.W. & Müller J. 2013: Current near-to-nature forest management effects on functional trait composition of saproxylic beetles in beech forests. - Conserv. Biol. 27: 605-614.
Go to original source... - Horwitz M. 2011: Saproxylic Coleoptera on Oak Trees (Quercus spp.) in the County of Norrtälje. A Study of Macro and Micro Habitat, Spatial Ecology and Species Composition of Saproxylic Beetles. Degree project in biology, Uppsala University, 37 pp.
- Hyvärinen E., Kouki J. & Martikainen P. 2006: A comparison of three trapping methods used to survey forest-dwelling Coleoptera. - Eur. J. Entomol. 103: 397-407.
Go to original source... - Katušić L., Jelaska S.D. & Šerić Jelaska L. 2017: Monitoring of saproxylic beetles in Croatia: following the path of the stag beetle. - Nat. Conserv. 19: 39-56.
Go to original source... - Koren T. 2017: The status and occurrence of Bolbelasmus unicornis Schrank, 1789 (Coleoptera: Geotrupidae) in Croatia. - Acta Zool. Bulg. 69: 139-142.
- Krebs C.J. 1998: Ecological Methodology. Harper & Row, New York, 654 pp.
- Krebs C.J. 1999: Ecological Methodology. Benjamin/Cummings, Menlo Park, CA, 620 pp.
- Krčmar S. 2014: List of insect fauna (Insecta) of Kopački Rit Nature Park (NE Croatia). - Türk. Entomol. Bül. 4: 15-39.
Go to original source... - Lachat T., Wermelinger B., Gossner M.M., Bussler H., Isacsson G., Müller J. & Bässler C. 2012: Saproxylic beetles as indicator species for dead-wood amount and temperature in European beech forests. - Ecol. Indic. 23: 323-331.
Go to original source... - Lauš B., Babić J. & Burić I. 2023a: Proposal for a Monitoring Programme for the Conservation Status of the Wrinkled Bark Beetle (Rhysodes sulcatus) in Croatia. OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. Hyla Association, Zagreb, 44 pp. [in Croatian].
- Lauš B., Babić J. & Burić I. 2023b: Proposal for a Monitoring Programme for the Conservation Status of Theunicorn Earth-borer Beetle (Bolbelasmus unicornis). OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. Hyla Association, Zagreb, 43 pp. [in Croatian].
- Lauš B., Ilinić M. & Burić I. 2023c: Proposal for a Monitoring Programme for the Conservation Status of the Violet Click Beetle (Limoniscus violaceus). OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. Hyla Association, Zagreb, 44 pp. [in Croatian].
- Lauš B., Ilinić M. & Burić I. 2023d: Proposal for a Monitoring Programme for the Conservation Status of the Flat Bark Beetle (Cucujus cinnaberinus). OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. Hyla Association, Zagreb, 45 pp. [in Croatian].
- Legendre P. & Legendre L. 2012: Numerical Ecology. 3rd ed. Elsevier, Amsterdam, 1006 pp.
- Litavský J., Majzlan O., Stašiov S., Svitok M. & Fedor P. 2021: The associations between ground beetle (Coleoptera: Carabidae) communities and environmental condition in floodplain forests in the Pannonian Basin. - Eur. J. Entomol. 118: 14-23.
Go to original source... - Martikainen P. & Kaila L. 2004: Sampling saproxylic beetles: lessons from a 10-year monitoring study. - Biol. Conserv. 120: 171-181.
Go to original source... - McCune B. & Grace J.B. 2002: PCORD Analysis of Ecological Communities. MJM Software Design, Gleneden Beach, OR, 305 pp.
- Mikšić R. 1965: Scarabaeidae of Yugoslavia. III. Scientific Society of Bosnia and Herzegovina, Publication No. 25, Sarajevo, 265 pp.
- Mikšić R. 1970: Katalog der Lamellicornia Jugoslawiens (Insecta - Coleoptera). Institute of Forestry, Sarajevo, 71 pp.
- Mikšić R. & Georgijević E. 1971: Cerambycidae of Yugoslavia. Part I. Book 3. Academy of Sciences and Arts of Bosnia and Herzegovina, Sarajevo, 175 pp.
- Mikšić R., Georgijević E. 1973: Cerambycidae of Yugoslavia. Part II. Book 4. Academy of Sciences and Arts of Bosnia and Herzegovina, Sarajevo, 153 pp.
- Mikšić R., Korpič M. 1985: Cerambycidae of Yugoslavia. Part III. Book 5. Academy of Sciences and Arts of Bosnia and Herzegovina, Sarajevo, 148 pp.
- Nieto A. & Alexander K.N.A. 2010: European Red List of Saproxylic Beetles. Publications Office of the EU, Luxembourg, 56 pp.
- Novák V. 2014: Beetles of the Family Tenebrionidae of Central Europe. Academia, Prague, 418 pp.
- Ranius T. & Jansson N. 2000: The influence of forest regrowth, original canopy cover and tree size on saproxylic beetles associated with old oaks. - Biol. Conserv. 95: 85-94.
Go to original source... - Ranius T. & Jansson N. 2002: A comparison of three methods to survey saproxylic beetles in hollow oaks. - Biodivers. Conserv. 11: 1759-1771.
Go to original source... - Rukavina I., Mrazović A., Kučinić M. & Šerić Jelaska L. 2010: Assemblage, zoogeography and endangered status of Carabid beetles in forest habitats of the Učka Nature Park. - Entomol. Croat. 14: 121-134.
- Rukavina I., Kostanjšek F., Jelaska S.D., Pirnat A. & Šerić Jelaska L. 2018: Distribution and habitat suitability of two rare saproxylic beetles in Croatia - a piece of puzzle missing for South-Eastern Europe. - iForest - Biogeosci. Forestry 11: 765-774.
Go to original source... - Šag M., Turić N. & Vignjević G. 2016: Saproxylic beetles in Croatian floodplain forests. - Entomol. Croat. 20: 45-67.
- Sánchez-Bayo F. & Wyckhuys K.A.G. 2019: Worldwide decline of the entomofauna: A review of its drivers. - Biol. Conserv. 232: 8-27.
Go to original source... - Schlaghamerský J., Maňák V. & Čechovský P. 2008: On the mass occurrence of two rare saproxylic beetles, Cucujus cinnaberinus (Cucujidae) and Dircaea australis (Melandryidae), in South Moravian floodplain forests. - Rev. Ecol. (Terre Vie) 10: 115-121.
Go to original source... - Seibold S., Bässler C., Brandl R., Gossner M.M., Thorn S., Ulyshen M.D. & Müller J. 2015: Experimental studies of dead-wood biodiversity - a review identifying global gaps in knowledge. - Biol. Conserv. 191: 139-149.
Go to original source... - Seibold S., Gossner M.M., Simons N.K., Blüthgen N., Müller J., Ambarli D., Ammer C., Bauhus J., Fischer M., Habel J.C. et al. 2019: Arthropod decline in grasslands and forests is associated with landscape-level drivers. - Nature 574: 671-674.
Go to original source... - Šerić Jelaska L. & Horvatić B. 2023a: Monitoring Programme for the Conservation Status of Lucanus cervus and Cerambyx cerdo. OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. PMF & Geonatura, Zagreb, 62 pp. [in Croatian].
- Šerić Jelaska L. & Horvatić B. 2023b: Monitoring Programme for the Conservation Status of Rosalia alpina. OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. PMF & Geonatura, Zagreb, 53 pp. [in Croatian].
- Šerić Jelaska L. & Horvatić B. 2023c: Monitoring Programme for the Conservation Status of Morimus funereus. OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. PMF & Geonatura, Zagreb, 51 pp. [in Croatian].
- Šerić Jelaska L. & Horvatić B. 2023d: Monitoring Programme for the Conservation Status of Osmoderma eremita Species Complex. OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. PMF & Geonatura, Zagreb, 52 pp. [in Croatian].
- Sláma M. 2006: Coleoptera: Cerambycidae, Icones Insectorum Europae Centralis. Kabourek, Zlín, 40 pp.
- Tischler W. 1949: Fundamentals of Terrestrial Animal Ecology. Vieweg & Braunschweig, Wiesbaden, 220 pp.
- Turić N. & Vignjević G. 2023: Proposal for a Monitoring Programme for the Conservation Status of Graphoderus bilineatus. OPKK project "Development of a system for monitoring the status of species and habitat types" - Group 11. Bioidea d.o.o., Osijek, 43 pp. [in Croatian].
- Turić N., Šag M., Kljajić K., Deže D., Batrnek K. & Bjelovuk D. 2019: Saproxylic beetle assemblages in Osijek parks. - Nat. Croat. 28: 89-97.
Go to original source... - Ulyshen M.D., Pawson S., Branco M., Horn S., Hoebeke E.R. & Gossner M.M. 2018: Utilization of non-native wood by saproxylic insects. In Ulyshen M.D. (ed.): Saproxylic Insects: Diversity, Ecology and Conservation. Springer, Cham, pp. 797-834.
Go to original source... - Vodka S., Konvička M. & Čížek L. 2009: Habitat preferences of oak-feeding xylophagous beetles in a temperate woodland: implications for forest history and management. - J. Insect Conserv. 13: 553-562.
Go to original source... - Vrezec A. 2008: Phenological estimation of imagos occurrence in four saproxylic beetle species of conservation importance in Slovenia: Lucanus cervus, Cerambyx cerdo, Rosalia alpina, Morimus funereus (Coleoptera: Lucanidae, Cerambycidae). - Acta Entomol. Slov. 16: 117-126.
- Vrezec A., Ambrožič Š. & Kapla A. 2012: An overview of sampling methods tests for monitoring schemes of saproxylic beetles in the scope of Natura 2000 in Slovenia. In Jurc M. (ed.): Saproxylic Beetles in Europe: Monitoring, Biology and Conservation. Slovenian Forestry Institute (Silva Slovenica; Studia Forestalia Slovenica), Ljubljana, pp. 79-90.
- Walther B.A. & Martin J.L. 2001: Species richness estimation of bird communities: How to control for sampling effort? - Ibis 143: 413-419.
Go to original source...
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.




ORCID...