Eur. J. Entomol. 121: 260-268, 2024 | DOI: 10.14411/eje.2024.027

Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stressOriginal article

Long-Chun HE ORCID...1, Jian-Yu MENG ORCID...2, Guy SMAGGHE ORCID...1, Chang-Li YANG ORCID...1, Xue TANG ORCID...1, Chang-Yu ZHANG ORCID...1, *
1 Institute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou University, Guiyang, Guizhou 550025, China; e-mails: helongchun0303@gmail.com, guysma9@gmail.com, yangchangli@126.com, aneira1994@163.com, gzuzcy1121@126.com
2 Guizhou Tobacco Science Research Institute, Guiyang, Guizhou 550081, China; e-mail: mengjy0417@163.com

Ultraviolet B (UV-B) is a significant environmental factor affecting insect development, survival, and reproduction. DNAJ proteins are molecular chaperones found ubiquitously in insects that are crucial for their adaptation to environmental stresses. This study aimed to elucidate the roles of DNAJ genes in the response of Myzus persicae to UV-B stress. Herein, we identified six DNAJ genes in the aphid M. persicae, a devastating agricultural pest. We analyzed their expression profiles at different stages of development, in different tissues and for various durations of UV-B exposure. The expression levels of MpDNAJC30, MpDNAJC11, MpDNAJC2, and MpDNAJC3 were highest in wingless adults, while MpDNAJC9 and MpDNAJC17 were highest in second- and third-instar nymphs, respectively. Six MpDNAJs had higher expression levels in the epidermis and embryos, and lower levels in the head. Additionally, the expression levels of all genes increased significantly under different durations of UV-B exposure. Knockout of the DNAJ genes using RNA interference caused a significant decline in the survival rate, weight, body length, and body width of M. persicae exposed to UV-B radiation. Our research provides valuable insights into the stress response mechanisms of M. persicae, highlighting the importance of DNAJ genes in mediating their adaptation to UV-B stress.

Keywords: Aphid, agricultural pest, heat shock proteins, RNAi, UV radiation stress

Received: May 17, 2024; Revised: June 19, 2024; Accepted: June 19, 2024; Published online: July 15, 2024  Show citation

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HE, L., MENG, J., SMAGGHE, G., YANG, C., TANG, X., & ZHANG, C. (2024). Identification and functional analysis of six DNAJ genes from Myzus persicae (Hemiptera: Aphididae) in response to UV-B stress. EJE, 121, Article 260-268. https://doi.org/10.14411/eje.2024.027
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