Eur. J. Entomol. 120: 93-104, 2023 | DOI: 10.14411/eje.2023.012

Locations of Rab, Allatotropin, Prothoracicotropic hormone and Period in the larval brain, corpus allatum and frontal ganglion of Bombyx mori Original article

Asuka MATSUI1, Makoto TOKUSHIGE1, Akira MIZOGUCHI2, Kengo KANAMARU1, Katsuhiko SAKAMOTO3, Yuichi UNO4, Tomohide UNO1, *
1 Department of Applied Chemistry in Bioscience Biological Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan; e-mails: 213a402a@stu.kobe-u.ac.jp, 202a428a@stu.kobe-u.ac.jp, kng@kobe-u.ac.jp, unotom@kobe-u.ac.jp
2 Division of Liberal Arts and Sciences, Aichi Gakuin University, Nisshin, Aichi 470-0195, Japan; e-mail: amizo@dpc.agu.ac.jp
3 Graduate School of Agricultural Science, Faculty of Agriculture, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan; e-mail: ksakamoto@kobe-u.ac.jp
4 Department of Plant Resource Science, Faculty of Agriculture, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan; e-mail: yuno@kobe-u.ac.jp

Rab, a low molecular weight GTP-binding protein, regulates the transmission of intracellular proteins. Insect neuropeptides that are directly involved in growth, development, reproduction, homeostasis, courtship, feeding, circadian rhythm and many other physiological processes are synthesized in neurons and ganglia in the brain and secreted by specific neurosecretory cells in tissues such as the corpus allatum and frontal ganglion. To clarify the relationship between Rabs, two neuropeptides, Allatotropin (AT) and Prothoracicotropic hormone (PTTH) and the circadian clock protein, Period (PER), were used to determine the locations of nine Rabs (Rab1, Rab3, Rab6, Rab7, Rab11, Rab14, Rab19, Rab21 and RabX4). Rab6-, Rab11-, Rab14- and Rab21-immunohistochemical reactivities (IRs) partially overlapped AT-IR in the brain in B. mori. Rab3-, Rab6-, Rab11-, Rab14-, and Rab21-IRs overlapped AT-IR in the frontal ganglion. Of the seven Rabs, only Rab11-IR overlapped PTTH-IR in the brain. Rab1-, Rab3-, Rab11-, Rab14-, Rab19-, Rab21-, RabX4- and NUF (nuclear fallout, an effector of Rab11)-IRs, overlapped PER-IR in the brain. Therefore, Rab may regulate the exocytosis of PTTH, AT and a protein associated with the circadian rhythm.

Keywords: Lepidoptera, Bombycidae, immunohistochemistry, silkworm, frontal ganglion, insect neurohormone, PTTH

Received: November 23, 2022; Revised: March 14, 2023; Accepted: March 14, 2023; Published online: March 29, 2023  Show citation

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MATSUI, A., TOKUSHIGE, M., MIZOGUCHI, A., KANAMARU, K., SAKAMOTO, K., UNO, Y., & UNO, T. (2023). Locations of Rab, Allatotropin, Prothoracicotropic hormone and Period in the larval brain, corpus allatum and frontal ganglion of Bombyx mori . EJE, 120, Article 93-104. https://doi.org/10.14411/eje.2023.012
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