Eur. J. Entomol. 114: 546-553, 2017 | DOI: 10.14411/eje.2017.069

Chromosome mapping of 28S ribosomal genes in 11 species of Cassidinae (Coleoptera: Chrysomelidae)

Amália T. LOPES1, Flávia R. FERNANDES2, Marielle C. SCHNEIDER3
1 Universidade Estadual Paulista, UNESP, Departamento de Biologia, Avenida 24A, 1515, Bela Vista, 13506-900, Rio Claro, São Paulo, Brasil; e-mail: amalia.torrezan@gmail.com
2 Museu Paraense Emílio Goeldi, MPEG, Departamento de Entomologia, Avenida Perimetral, 1901, Terra Firme, 66077-830, Belém, Para, Brasil; e-mail: flarfer@gmail.com
3 Universidade Federal de São Paulo, UNIFESP, Departamento de Ciências Biológicas, Avenida Professor Artur Riedel, 275, 09972-270, Diadema, São Paulo, Brasil; e-mail: marielle.unifesp@gmail.com

In this study, we examined for the first time the distribution of the 28S ribosomal genes in beetles of the subfamily Cassidinae. More than 55% of the species in this subfamily have a similar karyotype, 2n = 16 + Xyp. For this work, we selected species belonging to the tribes Cassidini and Mesomphaliini, which have, respectively, the most conserved and diversified karyotype characteristics within the Cassidinae. An analysis of 11 species revealed that rDNA sites on one pair of autosomes is the most frequent pattern, occurring in 10 species. This condition occurs in the seven genera examined and in species of both of the tribes, Cassidini and Mesomphaliini. Nevertheless, the differences in the locations of 28S rDNA were more pronounced in the tribe Cassidini and among species with similar karyotype characteristics. On the other hand, in Mesomphaliini, the increase in the diploid number was not accompanied by an increase in the number of ribosomal sites. Moreover, the comparison of the number and localization of major rDNA sites with the distribution of constitutive heterochromatin indicates that there is no direct correlation between the dispersion of constitutive heterochromatin and 28S rDNA genes in Cassidinae.

Keywords: Coleoptera, Chrysomelidae, Cassidini, Mesomphaliini, constitutive heterochromatin, diploid number, nucleolar organizer region, rDNA genes

Received: July 13, 2017; Accepted: October 23, 2017; Revised: October 23, 2017; Published online: December 7, 2017Show citation

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LOPES, A.T., FERNANDES, F.R., & SCHNEIDER, M.C. (2017). Chromosome mapping of 28S ribosomal genes in 11 species of Cassidinae (Coleoptera: Chrysomelidae). Eur. J. Entomol.114(1), 2017.000. doi: 10.14411/eje.2017.069.
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References

  1. Almeida M.C., Campaner C. & Cella D.M. 2006: Karyotype characterization, constitutive heterochromatin and nucleolus organizer regions of Paranaita opima (Coleoptera, Chrysomelidae, Alticinae). - Genet. Mol. Biol. 29: 475-481. Go to original source...
  2. Almeida M.C., Goll L.G., Artoni R.F., Nogaroto V., Matiello R.R. & Vicari M.R. 2010: Physical mapping of 18S rDNA cistron in species of the Omophoita genus (Coleoptera, Alticinae) using fluorescent in situ hybridization. - Micron 41: 729-734. Go to original source...
  3. Arcanjo A.M., Cabral-de-Mello D.C., Barros e Silva A.E. & Moura R.C. 2009: Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion. - J. Genet. 88: 177-182. Go to original source...
  4. Arcanjo A.M., Cabral-de-Mello D.C., Martins C., Moura R.C. & Souza M.J. 2013: Chromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae). - Genet. Mol. Biol. 36: 341-346. Go to original source...
  5. Bione E., Moura R.C., Carvalho R. & Souza M.J. 2005: Karyotype, C-and fluorescence banding pattern, NOR location and FISH study of five Scarabaeidae (Coleoptera) species. - Genet. Mol. Biol. 28: 376-381. Go to original source...
  6. Blackmon H. & Demuth J.P. 2015: Coleoptera karyotype database. - Coleopt. Bull. 69.1: 174-175. Go to original source...
  7. Bombarová M., Marec F., Nguyen P. & ©pakulová M. 2007: Divergent location of ribosomal genes in fish thorny-headed worms, Pomphorhynchus laevis and Pomphorhynchus tereticollis (Acanthocephala). - Genetica 131: 141-149. Go to original source...
  8. Cabral-de-Mello D.C., Moura R.C. & Martins C. 2010: Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement. - Heredity 104: 393-400. Go to original source...
  9. Cabral-de-Mello D.C., Oliveira S.G., Moura R.C. & Martins C. 2011a: Chromosomal organization of the 18S and 5S rRNAs and histone H3 genes in Scarabaeinae coleopterans: insights into the evolutionary dynamics of multigene families and heterochromatin. - BMC Genet. 12: 88, 12 pp. Go to original source...
  10. Cabral-de-Mello D.C., Moura R.C. & Martins C. 2011b: Cytogenetic mapping of rRNAs and histone H3 genes in 14 species of Dichotomius (Coleoptera, Scarabaeidae, Scarabaeinae) beetles. - Cytogenet. Genome Res. 134: 127-135. Go to original source...
  11. Cabrero J. & Camacho J.P. 2008: Location and expression of ribosomal RNA genes in grasshoppers: Abundance of silent and cryptic loci. - Chromos. Res. 16: 595-607. Go to original source...
  12. Chaboo C.S. 2007: Biology and phylogeny of the Cassidinae Gyllenhal sensu lato (tortoise and leaf-mining beetles) (Coleoptera: Chrysomelidae). - Bull. Am. Mus. Nat. Hist. No. 305, 250 pp. Go to original source...
  13. Charlesworth B., Sniegowski O. & Stephan W. 1994: The evolutionary dynamics of repetitive DNA in eukaryotes. - Nature 371: 215-220. Go to original source...
  14. Colomba M.S., Monteresino E., Vitturi R. & Zunino M. 1996: Characterization of mitotic chromosomes of the scarab beetles Glyphoderus sterquilinus (Westwood) and Bubas bison (L.) (Coleoptera: Scarabaeidae) using conventional and banding techniques. - Biol. Zbl. 115: 58-70.
  15. Colomba M.S., Vitturi R. & Zunino M. 2000a: Chromosome analysis and rDNA FISH in the stag beetle Dorcus parallelipipedus L. (Coleoptera: Scarabaeoidea: Lucanidae). - Hereditas 133: 249-253. Go to original source...
  16. Colomba M.S., Vitturi R. & Zunino M. 2000b: Karyotype analysis, banding, and fluorescent in situ hybridization in the scarab beetle Gymnopleurus sturmi McLeay (Coleoptera Scarabaeoidea: Scarabaeidae). - J. Hered. 91: 260-264. Go to original source...
  17. Colomba M., Vitturi R., Volpe N., Lannino A. & Zunino M. 2004: Karyotype, banding and rDNA FISH in the scarab beetle Anoplotrupes stercorosus (Coleoptera: Scarabaeoidea: Geotrupidae). Description and comparative analysis. - Micron 35: 717-720. Go to original source...
  18. Colomba M., Vitturi R., Libertini A., Gregorini A. & Zunino M. 2006: Heterochromatin of the scarab beetle, Bubas bison (Coleoptera: Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies. - Micron 37: 1-5. Go to original source...
  19. Costa C. 2003: Estado de conocimiento de los Coleoptera neotropicales. - Bol. SEA (Versión electrónica) No. 32. URL: http://www. sea-entomologia.org/aracnet/11/01/index.htm.
  20. Datson P.M. & Murray B.G. 2006: Ribosomal DNA locus evolution in Nemesia: transposition rather than structural rearrangement as the key mechanism? - Chromos. Res. 14: 845-857. Go to original source...
  21. De Julio M., Fernandes F.R., Costa C., Almeida M.C. & Cella D.M. 2010: Mechanisms ok karyotype differentiation in Cassidinae sensu latu (Coleoptera, Polyphaga, Chrysomelidae) based on seven species of Brazilian fauna and an overview of the cytogenetic data. - Micron 41: 26-38. Go to original source...
  22. De Vaio E.S. & Postiglioni A. 1974: Stolaine cassidinaes (Coleoptera, Chrysomelidae) with Xyp sex chromosomes and derivative system XpneoXneoYp. - Can. J. Genet. Cytol. 16: 433-440. Go to original source...
  23. Dutrillaux A.M. & Dutrillaux B. 2009: Chromosome polymorphism and Fanconi-like instability in the Scarabaeid beetle Macraspis tristisfrom Guadeloupe. - Cytogenet. Genome Res. 125: 142-148. Go to original source...
  24. Dutrillaux A.M. & Dutrillaux B. 2012: Chromosome analysis of 82 species of Scarabaeoidea (Coleoptera), with special focus on NOR localization. - Cytogenet. Genome Res. 136: 208-219. Go to original source...
  25. Dutrillaux A.M., Xie H. & Dutrillaux B. 2007: Nucleolus and chromosome relationships at pachynema in four Scarabaeoidea (Coleoptera) species with various combinations of NOR and sex chromosomes. - Chromos. Res. 15: 417-427. Go to original source...
  26. Dutrillaux A.M., Mercier J., Xie H. & Dutrillaux B. 2008: Etude chromosomique de 16 espèces ou sous-espèces de Cetoniini (Coleoptera: Scarabaeidae: Cetoniinae) d'Europe. - Ann. Soc. Entomol. Fr. 44: 443-450. Go to original source...
  27. Giannoulis T., Dutrillaux A.M., Mmamuris Z., Montreuil O., Stamatis C. & Dutrillaux B. 2011: Evolution of European cockchafers (Melolonthinae: Scarabaeidae: Coleoptera): a morphological, molecular and chromosomal study of intra- and interspecific variations. - Bul. Entomol. Res. 101: 345-352. Go to original source...
  28. Goll L.G., Artoni R.F. Vicari M.R., Nogaroto V., Petitpierre E. & Almeida M.C. 2013: Cytogenetic analysis of Lagria villosa (Coleoptera, Tenebrionidae): emphasis on the mechanism of association of the Xyp sex chromosomes. - Cytogenet. Genome Res. 139: 29-35. Go to original source...
  29. Goll L.G., Matiello R.R., Artoni R.F., Vicari M.R., Nogaroto V., Barros A.V. & Almeida M.C. 2015: High-resolution physical chromosome mapping of multigene families in Lagria villosa (Tenebrionidae): occurrence of interspersed ribosomal genes in Coleoptera. - Cytogenet. Genome Res. 146: 64-70. Go to original source...
  30. Gómez-Zurita J., Pons J. & Petitpierre E. 2004: The evolutionary origin of a novel karyotype in Timarcha (Coleoptera, Chrysomelidae) and general trends of chromosome evolution in the genus. - J. Zool. Sys. Evol. Res. 42: 332-341. Go to original source...
  31. Holecová M., Rozek M. & Lachowska D. 2008: The first cytogenetic report on Laena reitteri Weise, 1877 (Coleoptera, Tenebrionidae, Lagriinae) with notes on karyotypes of darkling beetles. - Folia Biol. (Kraków) 56: 213-217. Go to original source...
  32. Holecová M., Maryañska-Nadachowska A. & Rozek M. 2013: Cytogenetic analysis of Otiorhynchus bisulcatus (Fabricius, 1781) and O. (Zadrehus) atroapterus (De Geer, 1775) (Coleoptera, Curculionidae, Entiminae) using C Bands, NORs, and DAPI/CMA3 staining. - Folia Biol. (Kraków) 61: 177-183. Go to original source...
  33. Howell W.M. & Black D.A. 1980: Controlled silver staining of nucleolus organizer regions with protective colloidal developer: a 1-step method. - Experientia 36: 1014-1015. Go to original source...
  34. Karagyan G., Lachowska D. & Kalashian M. 2012: Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining. - Comp. Cytogenet. 6: 183-197. Go to original source...
  35. Lachowska D., Rozek M. & Holecová M. 2008: Cytotaxonomy and karyology of the tribe Otiorhynchini (Coleoptera: Curculionidae). - Eur. J. Entomol. 105: 175-184. Go to original source...
  36. Lira-Neto A.C., Silva G.M., Moura R.C. & Souza M.J. 2012: Cytogenetics of the darkling beetles Zophobas aff. confusus and Nyctobates gigas (Coleoptera, Tenebrionidae). - Genet. Mol. Res. 11: 2432-2440. Go to original source...
  37. Long E.O. & Dawid I.B. 1980: Repeated genes in Eucaryotes. - Annu. Rev. Biochem. 49: 727-764. Go to original source...
  38. Lopes A.T., Fernandes F.R. & Schneider M.C. 2016: Comparative cytogenetic analysis in 13 tortoise beetles (Cassidinae, Coleoptera) from Brazilian fauna. - Eur. J. Entomol. 113: 352-363. Go to original source...
  39. Mendes-Neto E.O., Vicari M.R., Campaner C., Nogaroto V., Artoni R.F. & Almeida M.C. 2010: Cytogenetic analysis of Astylus antis (Perty, 1830) (Coleoptera, Melyridae): karyotype, heterochromatin and location of ribosomal genes. - Genet. Mol. Biol. 33: 237-243. Go to original source...
  40. Moura R.C., Souza M.J., Melo N.F. & Lira-Neto A.C. 2003: Karyotypic characterization of representatives from Melolonthinae (Coleoptera: Scarabaeidae): karyotypic analysis, banding and fluorescent in situ hydridization (FISH). - Hereditas 138: 200-206. Go to original source...
  41. Moura R.C., Melo N.F. & Souza M.J. 2008: High levels of chromosomal differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae). - Genet. Mol. Biol. 31: 431-437. Go to original source...
  42. Nguyen P., Sahara K., Yoshido A. & Marec F. 2010: Evolutionary dynamics of rDNA clusters on chromosomes of moths and butterflies (Lepidoptera). - Genetica 138: 343-354. Go to original source...
  43. Nunn G. 1992: Nematode Molecular Evolution. An Investigation of Evolutionary Patterns among Nematodes Based upon DNA Sequences. PhD thesis, University of Nottingham, UK.
  44. Oliveira S.G., Moura R.C., Silva A.E.B. & Souza M.J. 2010: Cytogenetic analysis of two Coprophanaeus species (Scarabaeidae) revealing wide constitutive heterochromatin variability and the largest number of 45S rDNA sites among Coleoptera. - Micron 41: 960-965. Go to original source...
  45. Oliveira S.G., Moura R.C. & Martins C. 2012a: B chromosome in the beetle Coprophanaeus cyanescens (Scarabaeidae): emphasis in the organization of repetitive DNA sequences. - BMC Genet. 13: 96-101. Go to original source...
  46. Oliveira S.G., Cabral-de-Mello D.C., Arcanjo A.P., Xavier C., Souza M.J., Martins C. & Moura R.C. 2012b: Heterochromatin, sex chromosomes and rRNA gene clusters in Coprophanaeus beetles (Coleoptera, Scarabaeidae). - Cytogenet. Genome Res. 138: 46-55. Go to original source...
  47. Petitpierre E. 1970: Cytotaxonomy and evolution of Timarcha Latr. (Coleoptera: Chrysomelidae). - Genet. Iber. 22: 67-120.
  48. Petitpierre E. 1976: Further cytotaxonomical and evolutionary studies on the genus Timarcha Latr. (Coleoptera: Chrysomelidae). - Genet. Iber. 28: 57-81.
  49. Petitpierre E. 1996: Molecular cytogenetics and taxonomy of insects, with particular reference to the Coleoptera. - Int. J. Insect Morphol. Embryol. 25: 115-134. Go to original source...
  50. Pinkel D., Straume T. & Gray J.W. 1986: Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. - Proc. Natn. Acad. Sci. USA 83: 2934-2938. Go to original source...
  51. Postiglioni A. & Brum-Zorrilla N. 1988: Non-relationship between nucleolus and sex chromosome system Xyp in Chelymorpha variabilis Boheman (Coleoptera: Chrysomelidae). - Genetica 77: 137-141. Go to original source...
  52. Postiglioni A., Mazzella M.C., Panzera F., Da Silva A., Ponce de León R., Vasina L.K. & Scvortzoff E. 1990: Sex chromosomes of Neotropical Coleoptera from Uruguay. - Nucleus 33: 25-30.
  53. Postiglioni A., Stoll M. & Brum-Zorrila N. 1991: Haploid karyotype analysis of Chelymorpha variabilis Bohemam (Coleoptera, Chrysomelidae) with microspreading techiques. - Rev. Bras. Genet. 14: 653-660.
  54. Proença S.J.R., Serrano A.R.M., Serrano J. & Galián J. 2011: Patterns of rDNA chromosomal localization in Palearctic Cephalota and Cylindera (Coleoptera: Carabidae: Cicindelini) with different numbers of X-chromosomes. - Comp. Cytogenet. 5: 47-59. Go to original source...
  55. Rufas J.S., Iturra P., De Souza W. & Esponda P. 1982: Simple silver staining procedure for the localization of nucleolus and nucleolar organizers under light and electron microscopy. - Arch. Biol. Sci. 93: 267-276.
  56. Schneider M.C., Artoni R.F. & Almeida M.C. 2002: Cytogenetic analysis of 3 populations of Diabrotica speciosa (Chrysomelidae, Galerucinae): Constitutive heterochromatin and nucleolus organizer regions. - Cytologia 67: 281-287. Go to original source...
  57. Schneider M.C., Almeida M.C., Rosa S.P., Costa C. & Cella D.M. 2006: Evolutionary chromosomal differentiation among four species of Conoderus Eschscholtz, 1829 (Coleoptera, Elateridae, Agrypninae, Conoderini) detected by standard staining, C-banding, silver nitrate impregnation, and CMA3/DA/ DAPI staining. - Genetica 128: 333-346. Go to original source...
  58. Schneider M.C., Rosa S.P., Almeida M.C., Costa C. & Cella D.M. 2007: Chromosomal similarities and differences among four Neotropical Elateridae (Conoderini and Pyrophorini) and other related species, with comments on the NOR patterns in Coleoptera. - J. Zool. Syst. Evol. Res. 45: 308-316. Go to original source...
  59. Silva G.M., Bione E.G., Cabral-de-Mello D.C., Moura R.C., Simões Z.L.P. & Souza M.J. 2009: Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae). - Genet. Mol. Biol. 32: 276-280. Go to original source...
  60. Stevens N.M. 1906: Studies in spermatogenesis. II. A comparative study of the heterochromosomes in certain species of Coleoptera, Hemiptera, and Lepidoptera, with special reference to sex determination. - Carnegie I. Was. Pub. 36: 33-74.
  61. Vidal O.R. 1984: Chromosome numbers of Coleoptera from Argentina. - Genetica 65: 235-239. Go to original source...
  62. Virkki N. 1983: Banding of Oedionychina (Coleoptera, Alticinae) chromosomes C- and Ag-bands. - J. Agr. U. Puerto Rico 67: 221-255.
  63. Virkki N. & Denton A. 1987: Brief notes on the cytology of Neotropical Coleoptera. 4. Chalcolepidius silbermanni Chevrolat (Elateridae: Pyrophorini). - J. Agr. U. Puerto Rico 71: 323-326.
  64. Virkki N. & Sepúlveda J.M. 1990: Chromosomes of the Puerto Rican "vaquita", Diaprepes abbreviatus (L.) (Curculionidae: Otiorrhynchinae: Phyllobiini). - J. Agr. U. Puerto Rico 74: 121-132.
  65. Virkki N., Mazzella C. & Denton A. 1990: Staining of substances adjacent to the Xyp sex bivalent of some weevils (Coleoptera: Curculionidae). - J. Agr. U. Puerto Rico 74: 405-418.
  66. Virkki N., Santiago-Blay J.A. & Riley E.G. 1992: Chromosomes of Puerto Rican Hispinae and Cassidinae (Coleoptera: Chrysomelidae). - Coleopt. Bull. 46: 29-42.
  67. Vitturi R., Colomba M.S., Barbieri R. & Zunini M. 1999: Ribosomal DNA location in the scarab beetle Thorectes intermedius (Costa) (Coleoptera: Geotrupidae) using banding and fluorescent in-situ hybridization. - Chromos. Res. 7: 255-260. Go to original source...
  68. Vitturi R., Colomba M., Volpe N., Lannino A. & Zunino M. 2003: Evidence for male X0 chromosome system in Pentodon bidens punctatum (Coleoptera: Scarabaeoidea: Scarabaeidae) with X-linked 18S-28S rDNA clusters. - Genes Genet. Syst. 78: 427-432. Go to original source...
  69. Yadav J.S. & Pillai R.K. 1975: Karyological notes on four species of Cassidinae (Coleoptera: Chrysomelidae). - Genen Phaenen 18: 55-63.
  70. Yadav J.S., Vyas S. & Yadav A.S. 1992: Chromosome studies on five species of economically important beetles (Coleoptera). - Nucleus 35: 119-126.

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