Eur. J. Entomol. 108 (1): 161-163, 2011 | DOI: 10.14411/eje.2011.019

Highly polymorphic di- and trinucleotide microsatellite markers for the grapevine yellows disease vector Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae)

Miriam IMO1, Julia LÜNEBURG1, Thomas HANKELN2, Alfred SEITZ*,1, Jes JOHANNESEN1
1 Department of Ecology, Zoological Institute, University of Mainz, J.-J.-Becher-Weg 13, D-55128 Mainz, Germany; e-mail: imo@uni-mainz.de
2 Institute of Molecular Genetics, University of Mainz, J.-J.-Becher-Weg 30a, D-55128 Mainz, Germany

Seven polymorphic microsatellite loci were developed for the planthopper Hyalesthes obsoletus, vector of stolbur 16SrXII-A phytoplasma. The loci have di- or trinucleotide repeat motifs and are highly variable with 10 to 22 alleles per locus. Observed heterozygosity ranged from 0.278 to 0.950 for the 78 individuals genotyped. One locus is sex-linked. No linkage between loci was found. All loci amplified consistently among phylogeographic as well as host-plant related samples and proved highly informative for population genetic studies.

Keywords: Cixiidae, Hyalesthes obsoletus, Bois noir, microsatellite, PCR

Received: June 2, 2010; Accepted: July 28, 2010; Revised: July 28, 2010; Published: January 3, 2011Show citation

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IMO, M., LÜNEBURG, J., HANKELN, T., SEITZ, A., & JOHANNESEN, J. (2011). Highly polymorphic di- and trinucleotide microsatellite markers for the grapevine yellows disease vector Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae). Eur. J. Entomol.108(1), 161-163. doi: 10.14411/eje.2011.019.
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