Eur. J. Entomol. 103 (2): 291-296, 2006 | DOI: 10.14411/eje.2006.037

A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection

Yong Soo CHOI1, Kwang Sik LEE1, Hyung Joo YOON2, Iksoo KIM2, Hung Dae SOHN1, Byung Rae JIN*,1
1 College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea
2 Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Suwon 441-100, Korea

A thioredoxin-like protein (txl) gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus txl (Bitxl) gene spans 1777 bp and consists of three introns and four exons coding for 285 amino acid residues with a conserved active site (CGPC). The deduced amino acid sequence of the Bitxl cDNA was 65% similar to the Drosophila melanogaster txl. Northern blot analysis revealed the presence of Bitxl transcripts in all tissues examined. When H2O2 was injected into the body cavity of B. ignitus workers, Bitxl mRNA expression was up-regulated in the fat body tissue. In addition, the expression levels of Bitxl mRNA in the fat body greatly increased when B. ignitus workers were exposed to low (4°C) or high (37°C) temperatures, or injected with lipopolysaccharide (LPS), which suggests that the Bitxl possibly protects against oxidative stress caused by extreme temperatures and bacterial infection.

Keywords: Antioxidant enzyme, Apidae, bumblebee, Bombus ignitus, gene structure, oxidative stress, reactive oxygen species (ROS), thioredoxin-like protein (txl)

Received: August 10, 2005; Revised: September 12, 2005; Accepted: September 12, 2005; Published: April 6, 2006  Show citation

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Soo CHOI, Y., LEE, K.S., YOON, H.J., KIM, I., SOHN, H.D., & JIN, B.R. (2006). A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection. EJE, 103(2), 291-296. doi: 10.14411/eje.2006.037
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