Eur. J. Entomol. 102 (2): 289-291, 2005 | DOI: 10.14411/eje.2005.040

The use of RAPD markers to detect genetic patterns in Aleurodicus dispersus (Hemiptera: Aleyrodidae) populations from the Canary Islands

Carmen CALLEJAS1, Francisco J. BEITIA3, Antonio GOBBI2, Ana VELASCO1, M. Dolores OCHANDO*,1
1 Departamento de Genética, Facultad de CC. Biológicas, Universidad Complutense, Ciudad Universitaria, 28040 Madrid, Spain
2 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Apartado Oficial, 28080 Madrid, Spain
3 Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, 46113 Moncada (Valencia), Spain

Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae), a highly polyphagous species, has since the 90's been an important pest of ornamentals and tropical crops in the Canary Islands. In this study the RAPD-PCR technique was used to study the genetic structure of this whitefly in this archipelago. A total of 68 different bands were scored in seven populations using six primers for amplification. No differences in RAPD patterns were found among populations from different islands of the Canaries. These findings indicate a very high genetic similarity among populations and low level of genetic variability and support a single colonization event by few A. dispersus whiteflies and recent dispersion throughout the archipelago.

Keywords: Aleurodicus dispersus, spiralling whitefly, RAPD-PCR, genetic variability, colonization

Received: October 15, 2004; Revised: December 14, 2004; Accepted: December 14, 2004; Published: May 3, 2005  Show citation

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CALLEJAS, C., BEITIA, F.J., GOBBI, A., VELASCO, A., & OCHANDO, M.D. (2005). The use of RAPD markers to detect genetic patterns in Aleurodicus dispersus (Hemiptera: Aleyrodidae) populations from the Canary Islands. EJE, 102(2), 289-291. doi: 10.14411/eje.2005.040
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    References

    1. BEITIA F. 1998: Nueva especie de mosca blanca en las Islas Canarias. Terralia 4: 24-25
    2. HARRY M., ROBIN S. & LACHAISE D. 1998: L'utilisation de marqueurs genetiques polymorphes (RAPDs) en entomologie evolutive et appliquee. Ann. Entomol. Soc. Fr. 34: 9-32 Go to original source...
    3. HERNANDEZ-SUAREZ E. 1999: La familia Aleyrodidae y sus enemigos naturales en las Islas Canarias. PhD Thesis, Universidad de La Laguna, Tenerife, Islas Canarias, 687 pp
    4. HERNANDEZ-SUAREZ E., CARNERO A., HERNANDEZ M., BEITIA F. & ALONSO C. 1997: Lecanoideus floccissimus (Homoptera, Aleyrodidae): Nueva plaga en las Islas Canarias. Phytoma (Espana) 91: 35-48
    5. HIGUCHI R.G. 1989: Simple and rapid preparation of samples for PCR. In Erlich H.A. (ed.): PCR Technology. Stockton Press, New York, pp. 31-38 Go to original source...
    6. LIMA L.H.C., CAMPOS L., MORETZSOHN M.C., NAVIA D. & DE OLIVEIRA M.R.V. 2002: Genetic diversity of Bemisia tabaci (Genn.) populations in Brazil revealed by RAPD markers. Genet. Mol. Biol. 25: 217-223 Go to original source...
    7. MARTIN J.H., MIFSUD D. & RAPISARDA C. 2000: The whiteflies (Hemiptera: Aleyrodidae) of Europe and the Mediterranean Basin. Bull. Entomol. Res. 90: 407-448 Go to original source...
    8. MAYR E. 1963: Animal Species and Evolution. Harvard University Press, Cambridge, MA, 797 pp Go to original source...
    9. MOYA A., GUIRAO P., CIFUENTES D., BEITIA F. & CENIS J.L. 2001: Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera: Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction. Mol. Ecol. 10: 891-897 Go to original source...
    10. RUSSELL L.M. 1965: A new species of Aleurodicus Douglas and two close relatives (Homoptera, Aleyrodidae). Fla. Entomol. 48: 47-55 Go to original source...
    11. SIMON J.C., BAUMANN S., SUNNUCKS P., HEBERT P.D.N., PIERRE J.S., LE-GALLIC J.F. & DEDRYVER C.A. 1999: Reproductive mode and population genetic structure of the cereal aphid Sitobion avenae studied using phenotypic and microsatellite markers. Mol. Ecol. 8: 531-545 Go to original source...
    12. SHUFRAN K.A., BURD J.D. & WEBSTER J.A. 1997: Biotypic status of Russian wheat aphid (Homoptera: aphididae) populations in the United States. J. Econ. Entomol. 90: 1684-1689 Go to original source...
    13. WATERHOUSE D.J. & NORRIS K.R. 1989: Aleurodicus dispersus spiralling whitefly. In: Biological Control Pacific Prospects - Supplement 1. Australian Center for International Agriculture Research, Canberra, Australia, pp. 12-23
    14. YVON M. & PEROS J.P. 2003: Variation in aggressiveness and genetic diversity of grape phylloxera in southern France. J. Int. Sci. Vigne Vin 37: 77-84 Go to original source...

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