Eur. J. Entomol. 92 (1): 189-196, 1995
Ovary-specific interaction of ecdysone 22-phosphate with proteins in adult Drosophila melanogaster (Dipetra: Drosophilidae)
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Radiotracer experiments were performed to analyze the interaction of metabolites of ecdysone with proteins in adult Drosophila melanogaster females. [3H]Ecdysone was injected into the abdomen and after 4 h of incubation the flies were dissected. PBS-soluble material of the ovaries, haemolymph, gut and abdominal carcass were separated by gel-filtration. In all samples, two peaks containing radioactivity eluted with macromolecules (probably proteins) of an apparent molecular mass of more than 1,000 kDa (peak I) and about 500 kDa (peak II). In the extracts of the ovaries an additional peak in the molecular weight range of about 50 kDa (peak III) was detected. In all samples free ecdysone metabolites, which did not interact with macromolecules were detected. The radioactivity in the ovary-specific peak III was mainly due to ecdysone 22-phosphate. The high molecular weight peak I contained radioactive 22-fatty acyl esters of ecdysone and the radioactive material of peak II consisted of a highly complex mixture of ecdysteroids.
Keywords: Ecdysteroids, metabolism, binding proteins, gel-filtration, Drosophila melanogaster
Accepted: January 3, 1995; Published: February 24, 1995 Show citation
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