Eur. J. Entomol. 102 (2): 125-131, 2005 | DOI: 10.14411/eje.2005.020

Diagnostic molecular markers and the genetic relationships among three species of the Cheilosia canicularis group (Diptera: Syrphidae)

Vesna MILANKOV1, Jelena STAMENKOVIĆ1, Jasmina LUDOŠKI1, Gunilla STÅHLS2, Ante VUJIĆ1
1 Department of Biology and Ecology, University of Novi Sad, Trg Dositeja Obradoviüa 2, 21000 Novi Sad, Serbia and Montenegro; e-mail: vesnam@ib.ns.ac.yu
2 Finnish Museum of Natural History, PO Box 17, FIN-00014 University of Helsinki, Finland

To re-evaluate the taxonomic status of Cheilosia canicularis (Panzer, 1801), C. himantopus (Panzer, 1798) and C. orthotricha Vujić & Claussen, 1994, variation in the mitochondrial DNA (mtDNA) sequence of the cytochrome c oxidase subunit I (COI) gene and 18 nuclear allozyme genes were surveyed in allopatric and sympatric populations from Serbia and Montenegro. Genetic relationships among five populations of these species from the Fruška Gora (Serbia), Kopaonik (Serbia) and Durmitor (Montenegro) mountains were analyzed. Seven allozyme loci (Aat, Aco, Fum, Idh-1, Idh-2, Mdh-2 and Sdh) were diagnostic for delineating C. orthotricha from the other two species, while only a low, but consistent, genetic differentiation was observed between C. canicularis and C. himantopus. Differentiating all three species was possible based solely on the species-specific alleles at the Est-? locus. Sequence comparisons of 738 bp of the COI gene from eleven specimens was consistent with the variability in nuclear allozymes. Sequence data revealed variation in 5% of the nucleotide sites among C. orthotricha and the C. canicularis/C. himantopus pair, while less variation (0.68%) was observed within the pair C. canicularis/C. himantopus. However, the presence of one diagnostic allozyme locus and five consistently variable nucleotide sites in sympatric populations of C. canicularis and C. himantopus (Durmitor, Montenegro) suggest that these two species have separate gene pools.

Keywords: Allozymes, mtDNA, genetic differentiation, genetic variability, Cheilosia, canicularis, Cheilosia himantopus, Cheilosia orthotricha

Received: December 1, 2004; Revised: January 29, 2005; Accepted: January 29, 2005; Published: May 3, 2005  Show citation

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MILANKOV, V., STAMENKOVIĆ, J., LUDOŠKI, J., STÅHLS, G., & VUJIĆ, A. (2005). Diagnostic molecular markers and the genetic relationships among three species of the Cheilosia canicularis group (Diptera: Syrphidae). EJE, 102(2), 125-131. doi: 10.14411/eje.2005.020
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