Eur. J. Entomol. 101 (3): 419-422, 2004 | DOI: 10.14411/eje.2004.058

In vitro rearing of Anagrus breviphragma (Hymenoptera: Mymaridae), an egg parasitoid of Cicadella viridis (Hemiptera: Cicadellidae), from second instar larva to adult on diets without insect components

Elisabetta CHIAPPINI1, Maria Luisa DINDO2, Ilaria NEGRI1, Luca SIGHINOLFI2
1 Istituto di Entomologia e Patologia vegetale, Universita Cattolica del Sacro Cuore, via Emilia Parmense 84, 29100 Piacenza, Italy; e-mail: elisabetta.chiappini@unicatt.it
2 Dipartimento di Scienze e Tecnologie Agroambientali (Entomologia), viale Fanin 42, 40127 Bologna, Italy

We describe here the in vitro rearing of Anagrus breviphragma Soyka, an egg parasitoid of Cicadella viridis (Linnaeus), from the second instar larva to the adult stage. Three media, containing mainly either a commercial tissue culture medium (IPL-41), skimmed milk or veal homogenate, were tested. Larval development occurred on all the diets but few larvae reached the pupal stage on the diets containing skimmed milk or veal homogenate. On the tissue culture medium, pharate adults, albeit malformed, developed. Supplementing the tissue culture medium-based diet with chicken egg yolk alone, or combined with yeast extract, further improved parasitoid development. The addition of both egg yolk and yeast extract resulted in twelve out of twenty larvae reaching the adult stage, of which only four females emerged.

Keywords: Anagrus breviphragma, Mymaridae, Cicadella viridis, egg parasitoid, rearing, artificial diets

Received: September 10, 2003; Revised: April 9, 2004; Accepted: May 21, 2004; Published: September 20, 2004  Show citation

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CHIAPPINI, E., DINDO, M.L., NEGRI, I., & SIGHINOLFI, L. (2004). In vitro rearing of Anagrus breviphragma (Hymenoptera: Mymaridae), an egg parasitoid of Cicadella viridis (Hemiptera: Cicadellidae), from second instar larva to adult on diets without insect components. EJE, 101(3), 419-422. doi: 10.14411/eje.2004.058
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