Eur. J. Entomol. 101 (2): 211-218, 2004

Cytogenetic studies on Mepraia gajardoi (Heteroptera: Reduviidae). Chromosome behaviour in a spontaneous translocation mutant

Ruben PÉREZ1, Lucia CALLEROS1, Virginia ROSE1, Myriam LORCA2, Francisco PANZERA1
1 Sección Genética Evolutiva, Departamento de Biología Animal, Instituto de Biología, Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay; e-mail: rperez@fcien.edu.uy
2 Departamento de Parasitología, Facultad de Medicina, Universidad de Chile, P.O. Box 33052-33, Las Palmeras 299, Santiago de Chile, Chile

We analysed the chromosome complement and male meiosis in a natural population of Mepraia gajardoi (Hemiptera: Reduviidae: Triatominae). The normal karyotype of this species is 20 autosomes plus X1X2Y in males and X1X1X2X2 in females. We confirmed that M. gajardoi is cytogenetically strikingly different from M. spinolai (with which it was previously included), which supports the taxonomic separation of these two species. For the first time in the holocentric chromosomes of the subfamily Triatominae, a structural rearrangement was detected. Observations made during meiosis and mitosis indicated that one of eleven individuals had undergone a fusion between two non-homologous chromosomes, probably before germinal cell development. This autosomal translocation resulted in chromosomal irregularities such as an autosomal trivalent, autosomal univalents and chromosomal fragments, which altered the normal segregation of both autosomes and sex chromosomes. The chromosomal fragments resemble supernumerary or B chromosomes, which supports the idea that these chromosomes are remnants of structural rearrangements. The genetic consequences of translocations in holokinetic chromosomes and their role in the evolution of chromosomes in triatomines are also discussed.

Keywords: Heteroptera, Triatominae, Mepraia gajardoi, holocentric chromosome, autosomal translocation

Received: October 20, 2003; Revised: January 6, 2004; Accepted: February 2, 2004; Published: June 25, 2004  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
PÉREZ, R., CALLEROS, L., ROSE, V., LORCA, M., & PANZERA, F. (2004). Cytogenetic studies on Mepraia gajardoi (Heteroptera: Reduviidae). Chromosome behaviour in a spontaneous translocation mutant. EJE, 101(2), 211-218
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bressa M.J., Papeschi A.G., Mola L. & Larramendy M.L. 1998: Meiotic studies in Largus rufipenis (Castelnau) (Largidae, Heteroptera). II. Reciprocal traslocation heterozygosity. Caryologia 51: 253-264 Go to original source...
    2. Bressa M.J., Papeschi A.G., Mola L. & Larramendy M.L. 2001: Autosomal univalents as a common meiotic feature in Jadera haematoloma and Jadera sanguinolenta (Heteroptera: Rhopalidae: Serinethinae). Eur. J. Entomol. 98: 151-157 Go to original source...
    3. Camacho J.P., Sharbel T.F. & Beukeboom L.W. 2000: B-chromosome evolution. Philos. Trans. R. Soc. Lond. (B) (Biol. Sci.) 355: 163-178 Go to original source...
    4. Comings D.E & Okada T.A. 1972: Holocentric chromosomes in Oncopeltus: kinetochore plates are present in mitosis but absent in meiosis. Chromosoma 37: 177-192 Go to original source...
    5. Dujardin J.P., Schofield C.J. & Panzera F. 2002: Los Vectores de la Enfermedad de Chagas. Academie Royale des Sciences d'Outre-Mer, Classe des Sciences naturelles et medicales N.S., 25 (3), Bruxelles, 189 pp
    6. Frias D. & Atria J. 1998: Chromosomal variation, macroevolution and posible parapatric speciation in Mepraia spinolai (Porter) (Hemiptera: Reduviidae). Genet. Mol. Biol. 21(2): 179-184 Go to original source...
    7. Frias D.A., Henry A.A. & Gonzalez C.R. 1998: Mepraia gajardoi: a new species of Triatominae (Hemiptera: Reduviidae) from Chile and its comparison with Mepraia spinolai. Rev. Chil. Hist. Nat. 71: 177-188
    8. Hughes-Schrader S. & Schrader F. 1961: The kinetochore of the Hemiptera. Chromosoma 12: 327-350 Go to original source...
    9. Jurberg J., Lent H., Carcavallo R.U., Silva Rocha D., Galvao C. & Frias Lasserre D. 2002: Estudo morfologico da genitalia externa masculina de Mepraia gajardoi Frias, Henry & Gonzalez 1998, (Hemiptera. Reduviidae) com comentarios sobre suas relacoes filogeneticas. Entomol. Vect. 9: 559-577
    10. Manna G.K. 1984: Chromosomes in evolution in Heteroptera. In: A.K. Sharma A. & Sharma A. (eds): Chromosomes in Evolution of Eukaryotic groups. No. 2. CRC Press, Florida, pp. 189-225
    11. Nokkala S. 1986a: The mechanisms behind the regular segregation of the m-chromosomes in Coreus marginatus L. (Coreidae, Hemiptera). Hereditas 105: 73-85 Go to original source...
    12. Nokkala S. 1986b: The meiotic behaviour of B-chromosomes and their effect on the segregation of sex chromosomes in males of Hemerobius marginatus L. (Hemerobidae, Neuroptera). Hereditas 105: 221-227 Go to original source...
    13. Panzera F., Alvarez F., Sanchez-Rufas J., Perez R., Suja J., Scvortzoff E., Estramil E., Dujardin J.P. & Salvatella R. 1992: C-heterochromatin polymorphism in holocentric chromosomes of Triatoma infestans (Hemiptera-Reduvidae). Genome 35: 1068-1074 Go to original source...
    14. Panzera F., Perez R., Panzera Y., Alvarez F., Scvortzoff E. & Salvatella R. 1995: Karyotype evolution in holocentric chromosomes of three related species of triatomines (Hemiptera-Reduviidae). Chromosome Res. 3: 143-150 Go to original source...
    15. Panzera F., Hornos S., Pereira J., Cestau R., Canale D., Diotaiuti L., Dujardin J.P. & Perez R. 1997: Genetic variability and geographic differentiation among three species of triatomine bugs (Hemiptera-Reduviidae). Am. J. Trop. Med. Hyg. 57: 732-739 Go to original source...
    16. Panzera F., Scvortzoff E., Perez R., Panzera Y., Hornos S., Cestau R., Nicolini P., Delgado V., Alvarez F., Mazzella M., Cossio G., Martinez M. & Salvatella R. 1998: Citogenetics of Triatomines. In Atlas de Triatominos de America. Chapter 15. Fundacion Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil, pp. 621- 664
    17. Papeschi A.G. & Mola L.M. 1990: Meiotic studies in Acanonicus hahni (Stal) (Coreidae, Heteroptera). II. Male Chromosome behaviour in a spontaneous inversion mutant. Genetica 81: 59-66 Go to original source...
    18. Perez R., Panzera Y., Scafiezzo S., Mazzella M., Panzera F., Dujardin J.P. & Scvortzoff E. 1992: Cytogenetic as a tool for triatominae species distinction. Mem. Inst. Oswaldo Cruz 87: 353-361 Go to original source...
    19. Perez R., Panzera F., Page J., Suja J. & Rufas J. 1997: Meiotic behaviour of holocentric chromosomes: orientation and segregation of autosomes in Triatoma infestans (Heteroptera). Chromosome Res. 5: 47-56 Go to original source...
    20. Perez R., Hernandez M., Caraccio M.N., Rose V., Valente A., Valente V., Moreno J., Angulo V., Sandoval M., Roldan J., Vargas F., Wolff M. & Panzera F. 2002: Chromosomal Evolution Trends of the genus Panstrongylus (Hemiptera, Reduviidae), vectors of Chagas Disease. Infect. Genet. Evol. 2: 47-56 Go to original source...
    21. Schofield C.J., Apt W., Sagua H., Panzera F. & Dujardin J.P. 1998: Alary polymorphism in Triatoma spinolai and its possible relationship with demographic strategy. Med. Vet. Entomol. 12: 30-38 Go to original source...
    22. Toledo Piza S. 1944: A case of spontaneous end to end permanent union of two non homologous chromosomes in the Brazilian scorpion Tityus bahiensis accompanied by irregularities in pairing. Rev. Agric. 19: 133-147
    23. Ueshima N. 1963: Chromosome behaviour of the Cimex pilosellus complex (Cimicidae: Hemiptera). Chromosoma 14: 511-521 Go to original source...
    24. Ueshima N. 1966: Cytotaxonomy of the Triatominae (Reduviidae: Hemiptera). Chromosoma 18: 97-122 Go to original source...
    25. Ueshima N. 1979: Hemiptera II: Heteroptera. In John B. (ed.): Animal Cytogenetics 3. Insecta 6. Gebruder Borntrager, Berlin, 117 pp
    26. White M.J.D. 1973: Animal Cytology and Evolution. 3rd ed. Cambridge University Press, London, 961 pp
    27. WHO 2002: Control of Chagas Disease. Second report of the WHO Expert Committee. World Health Organization. Geneva. World Health Organ. Tech. Rep. Ser. 905: 1-109

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content