Eur. J. Entomol. 101 (1): 29-35, 2004 | DOI: 10.14411/eje.2004.007

The kind of AKH-mobilized energy substrates in insects can be predicted without a knowledge of the hormone structure

Radomír SOCHA1, Dalibor KODRÍK1,2, Petr ŠIMEK1, Markéta PATOČKOVÁ1,2
1 Institute of Entomology, Academy of Sciences, and
2 Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, České Budějovice, 370 05, Czech Republic; e-mail: socha@entu.cas.cz

The aim of this study was to show that the kind of AKH-mobilized energy substrates in insects can be predicted on the basis of the results obtained with the application of heterologous, i.e. inter-species, AKHs. Four different AKHs, the Locmi-AKH-I inducing hyperlipaemia and hyperglycaemia in Locusta migratoria, Tenmo-HrTH inducing hyperglycaemia in Tenebrio molitor, and Pyrap-AKH and Peram-CAH-II inducing hyperlipaemia in Pyrrhocoris apterus were used, firstly in conspecific tests, secondly in all possible species-AKH combinations, and finally in individual applications on the test species, the cotton bug Dysdercus cingulatus. Since each of the AKHs induced hyperlipaemia in D. cingulatus adults, we predicted that lipids are the only energy substrates which are mobilized in this species by its native AKH. The accuracy of this prediction was subsequently confirmed by the structural identification of the native D. cingulatus AKH and conspecific application tests. The proposed methodical approach can serve as a suitable monitoring system for determination of the kind of energy substrates mobilized by native insect AKHs until the structure of the hormone is identified.

Keywords: Adipokinetic hormones, Dysdercus cingulatus, Pyrrhocoris apterus, Locusta migratoria, Tenebrio molitor, Pyrap-AKH, lipids, carbohydrates, structure-function relations

Received: November 4, 2003; Revised: December 12, 2003; Accepted: January 22, 2004; Published: March 25, 2004  Show citation

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SOCHA, R., KODRÍK, D., ŠIMEK, P., & PATOČKOVÁ, M. (2004). The kind of AKH-mobilized energy substrates in insects can be predicted without a knowledge of the hormone structure. EJE, 101(1), 29-35. doi: 10.14411/eje.2004.007
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