Eur. J. Entomol. 94 (4): 553-555, 1997

Hatching ability of Bombyx mori (Lepidoptera: Bombycidae) eggs micro-injected with different concentrations of DNA at selected embryonic stages

SHAMILA Y., MATHAVAN S.
N/A

The hatching ability of Bombyx mori eggs subjected to microinjection of about 30 nl plasmid DNA (10 µg/ml) at 1-30 h of embryonic development and eggs injected at 8 h with DNA concentrations (0.5 to 500 µg/ml), averaged to about 22%. In respect to hatching ability, the optimum stage for microinjection is the preblastoderm (8-10 h old eggs) and the DNA concentration up to 10 µg/ml.

Keywords: Bombyx mori, microinjection, DNA concentration, embryonic stage, hatching ability

Accepted: November 6, 1996; Published: October 1, 1997  Show citation

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SHAMILA, Y., & MATHAVAN, S. (1997). Hatching ability of Bombyx mori (Lepidoptera: Bombycidae) eggs micro-injected with different concentrations of DNA at selected embryonic stages. EJE94(4), 553-555
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