Eur. J. Entomol. 96 (3): 309-315, 1999
Structure elucidation of Mas-AKH as the major adipokinetic hormone in the butterfly Vanessa cardui (Lepidoptera: Nymphalidae)
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The presence of adipokinetic activity in crude extracts of corpora cardiaca (CC) from the butterfly (Vanessa cardui L., Nymphalidae) was demonstrated by bioassay and Mas-AKH was revealed as the major adipokinetic hormone (AKH) by use of two different technologies of sequence elucidation: HPLC separation of the peptide followed by Edman degradation and Q-TOF mass spectrometry. In contrast to the time- and material-consuming conventional methods of peptide purification and sequencing, substantial structural data of the peptide were confirmed - post factum - from one pCC (pair of CC) by Q-TOF mass spectrometry. Only males of our laboratory colony showed a significant lipid increase in the haemolymph after injection of either crude CC extract (1 pCC equivalent) or 10 pmol of synthetic peptide.
Keywords: Adipokinetic hormone, Mas-AKH, butterfly, Nymphalidae, Vanessa cardui, lipid mobilization, Q-TOF mass spectrometry
Accepted: April 9, 1999; Published: October 25, 1999 Show citation
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