Eur. J. Entomol. 104 (4): 685-691, 2007 | DOI: 10.14411/eje.2007.086

Crustacean red pigment-concentrating hormone Panbo-RPCH affects lipid mobilization and walking activity in a flightless bug Pyrrhocoris apterus (Heteroptera) similarly to its own AKH-peptides

Radomír SOCHA1, Dalibor KODRÍK1,2, Rostislav ZEMEK1
1 Biology Centre ASCR, Institute of Entomology, Branišovská 31, CZ-370 05 České Budějovice, Czech Republic; e-mail: socha@entu.cas.cz
2 Faculty of Biological Sciences, South Bohemian University, Branišovská 31, České Budějovice, Czech Republic

In the present study we tested whether the walking activity of macropterous females of the flightless wing-polymorphic bug Pyrrhocoris apterus (L.) can be stimulated by its native adipokinetic hormone Peram-CAH-II and the crustacean red pigment-concentrating hormone (Panbo-RPCH), and the effectiveness of the latter hormone in a lipid mobilization assay. Two different doses (10 or 40 pmol) of Peram-CAH-II or Panbo-RCPH were injected into 10-day-old macropterous females of P. apterus to evaluate their effects on the walking activity of treated females. The results obtained showed a significant stimulation of walking activity only with the lower dose (10 pmol) of either hormone Peram-CAH-II or Panbo-RPCH. On the contrary, the walking activity of the same-aged females of macropterous morph treated with the higher dose (40 pmol) of these hormones was decreased. The energy substrates mobilized in Panbo-RPCH-treated macropterous females were lipids. The question of whether the stimulation of locomotion by Panbo-RPCH is limited only to P. apterus or if it might also represent an important function of this hormone in other insects or even in crustaceans is discussed.

Keywords: Adipokinetic hormone, Panbo-RPCH, Peram-CAH-II, locomotion, lipid mobilization, females, flightless, macropterous, Pyrrhocoris apterus

Received: March 14, 2007; Revised: May 14, 2007; Accepted: May 14, 2007; Published: October 15, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
SOCHA, R., KODRÍK, D., & ZEMEK, R. (2007). Crustacean red pigment-concentrating hormone Panbo-RPCH affects lipid mobilization and walking activity in a flightless bug Pyrrhocoris apterus (Heteroptera) similarly to its own AKH-peptides. EJE, 104(4), 685-691. doi: 10.14411/eje.2007.086
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. BRAY M.M., SHAFI S., WHEELER C.H. & GOLDSWORTHY G.J. 1993: Quantification by radioimmunoassay of the adipokinetic hormone-I in neural tissues in the head of Locusta migratoria. Comp. Biochem. Physiol. (A) 106: 257-262 Go to original source...
    2. FERNLUND P. & JOSEFSSON L. 1972: Crustacean color-change hormone: Amino acid sequence and chemical synthesis. Science 177: 173-175. Go to original source...
    3. GADE G. & GOLDSWORTHY G.J. 2003: Insect peptide hormones: a selective review of their physiology and potential application for pest control. Pest Manag. Sci. 59: 1063-1075 Go to original source...
    4. GADE G. & MARCO H.G. 2006: Structure, function and mode of action of selected arthropod neuropeptides. In Atta-urRahman (ed.): Studies in Natural Products Chemistry. Vol. 33. Elsevier, Amsterdam, pp. 69-139 Go to original source...
    5. GADE G., HOFFMANN K.H. & SPRING J.H. 1997: Hormonal regulation in insects: facts, gaps, and future directions. Physiol. Rev. 77: 963-1032 Go to original source...
    6. GADE G., AUERSWALD L., SIMEK P., MARCO H.G. & KODRIK D. 2003: Red pigment concentrating hormone is not limited to crustaceans. Biochem. Biophys. Res. Comm. 309: 972-978 Go to original source...
    7. GADE G., AUERSWALD L., PREDEL R. & MARCO H.G. 2004: Substrate usage and its regulation during flight and swimming in the backswimmer, Notonecta glauca. Physiol. Entomol. 29: 84-93 Go to original source...
    8. GADE G., AUERSWALD L. & MARCO H.G. 2006: Flight fuel and neuropeptidergic control of fuel mobilisation in the twig wilter, Holopterna alata (Hemiptera, Coreidae). J. Insect Physiol. 52: 1171-1181 Go to original source...
    9. GOLDSWORTHY G.J. 1983: The endocrine control of flight metabolism in locusts. Adv. Insect Physiol. 17: 149-204 Go to original source...
    10. GOLDSWORTHY G.J., JUTSUM A.R. & ROBINSON N.L. 1979: Substrate utilization and flight speed during tethered flight in the locust. J. Insect Physiol. 25: 183-185 Go to original source...
    11. GOLDSWORTHY G.J., KODRIK D., COMLEY R. & LIGHTFOOT M. 2002: A quantitative study of the adipokinetic hormone of the firebug, Pyrrhocoris apterus. J. Insect Physiol. 48: 1103-1109 Go to original source...
    12. HOLWERDA D.A., VAN DOORN J. & BEENAKKERS A.M.T. 1977: Characterization of the adipokinetic and hypertrehalosaemic substances from the locust corpus cardiacum. Insect Biochem. 7: 151-157 Go to original source...
    13. ISABEL G., MARTIN J.R., CHIDAMI S., VEENSTRA J.A. & ROSAY P. 2004: AKH-producing neuroendocrine cell ablation decreases trehalose and induces behavioural changes in Drosophila. Am. J. Physiol. (Regul. Integr. Comp. Physiol.) 288: 531-538 Go to original source...
    14. KODRIK D., SOCHA R., SIMEK P., ZEMEK R. & GOLDSWORTHY G.J. 2000: A new member of the AKH/RPCH family that stimulates locomotory activity in the firebug, Pyrrhocoris apterus (Heteroptera). Insect Biochem. Molec. Biol. 30: 489-498 Go to original source...
    15. KODRIK D., SOCHA R. & ZEMEK R. 2002a: Topical application of Pya-AKH stimulates lipid mobilization and locomotion in the flightless bug, Pyrrhocoris apterus (L.) (Heteroptera). Physiol. Entomol. 27: 15-20 Go to original source...
    16. KODRIK D., SIMEK P., LEPSA L. & SOCHA R. 2002b: Identification of the cockroach neuropeptide Pea-CAH-II as a second adipokinetic hormone in the firebug Pyrrhocoris apterus. Peptides 23: 583-585 Go to original source...
    17. KODRIK D., SOCHA R. & SYROVA Z. 2003: Developmental and diel changes of adipokinetic hormone in CNS and haemolymph of the flightless wing-polymorphic bug, Pyrrhocoris apterus. J. Insect Physiol. 49: 53-61 Go to original source...
    18. KODRIK D., SOCHA R., SYROVA Z. & ZEMEK R. 2005: The effect of constant darkness on the content of adipokinetic hormone, adipokinetic response and walking activity in macropterous females of Pyrrhocoris apterus (L.). Physiol. Entomol. 30: 248-255 Go to original source...
    19. LEE G. & PARK J.H 2004: Hemolymph sugar homeostasis and starvation-induced hyperactivity affected by genetic manipulations of the adipokinetic hormone-encoding gene in Drosophila melanogaster. Genetics 167: 311-323 Go to original source...
    20. LORENZ M.W., ZEMEK R., KODRIK D. & SOCHA R. 2004: Lipid mobilisation and locomotor stimulation in Gryllus bimaculatus (de Geer) (Ensifera, Gryllidae) by topically applied adipokinetic hormone. Physiol. Entomol. 29: 146-151 Go to original source...
    21. MAXOVA A., KODRIK D., ZEMEK R. & SOCHA R. 2001: Diel changes in adipokinetic response and walking activity of Pyrrhocoris apterus (L.) (Heteroptera) in relation to physiological status and wing dimorphism. Eur. J. Entomol. 98: 433-438 Go to original source...
    22. MILBURN N.S. & ROEDER K.D. 1962: Control of efferent activity in the cockroach terminal abdominal ganglion by extracts of corpora cardiaca. Gen. Comp. Endocrinol. 2: 70-76 Go to original source...
    23. MILDE J.J., ZIEGLER R. & WALLSTEIN M. 1995: Adipokinetic hormone stimulates neurons in the insect central nervous system. J. Exp. Biol. 198: 1307-1311 Go to original source...
    24. MOSHITZKY P., HENZEL W.J., RAFAELI A., RAMACHANDRAN J. & APPLEBAUM S.W. 1987a: Synthesis of adipokinetic hormone (AKH-I) in the locust brain. Insect Biochem. 17: 1133-1137 Go to original source...
    25. MOSHITZKY P., YAMASHIRO D., STUVE L., RAMACHANDRAN J. & APPLEBAUM S.W. 1987b: Determination of locust AKH-I by radioimmunoassay and the identification of an AKH-like factor in the locust brain. Insect Biochem. 17: 765-769 Go to original source...
    26. NASSEL D. 2002: Neuropeptides in the nervous system of Drosophila and other insects: multiple roles as neuromodulators and neurohormones. Prog. Neurobiol. 68: 1-84 Go to original source...
    27. OUDEJANS R.C.H.M., VROEMEN S.F., JANSEN R.F.R. & VAN DER HORST D.J. 1996: Locust adipokinetic hormones: carrierindependent transport and differential inactivation at physiological concentrations during rest and flight. Proc. Nat. Acad. Sci. USA 93: 8654-8659 Go to original source...
    28. SAS Inst. 2000: The SAS System for Linux, Release 8.2. SAS Onlinedoc. Version 8. Cary, NC, USA: SAS Institute Inc
    29. SCARBOUROUGH R.M., JAMIESON G.C., KALISH F., KRAMER S.J., MCENROE G.A., MILLER C.A. & SCHOOLEY D.A. 1984: Isolation and primary structure of two peptides with cardioacceleratory and hyperglycemic activity from the corpora cardiaca of Periplaneta americana. Proc. Natl. Acad. Sci. USA 81: 5575-5579 Go to original source...
    30. SCHOONEVELD H., ROMBERG-PRIVEE H.M. & VEENSTRA J.A. 1985: Adipokinetic hormone-immunoreactive peptide in the endocrine and central nervous system of several insect species: a comparative immunocytochemical approach. Gen. Comp. Endocr. 57: 184-194 Go to original source...
    31. SOCHA R. 1993: Pyrrhocoris apterus (Heteroptera) - an experimental model species: a review. Eur. J. Entomol. 90: 241-286
    32. SOCHA R. & KODRIK D. 1999: Differences in adipokinetic response of Pyrrhocoris apterus (Heteroptera) in relation to wing dimorphism and diapause. Physiol. Entomol. 24: 278-284 Go to original source...
    33. SOCHA R. & SULA J. 1996: Differences in haemolymph proteins in relation to diapause and wing dimorphism in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae). J. Comp. Physiol. (B) 166: 382-376 Go to original source...
    34. SOCHA R. & SULA J. 2006: Flight muscles polymorphism in a flightless bug, Pyrrhocoris apterus (L.): Developmental pattern, biochemical profile and endocrine control. J. Insect Physiol. 52: 231-239 Go to original source...
    35. SOCHA R. & ZEMEK R. 2000a: Wing movement behavior in longand short-winged morphs of flightless bug Pyrrhocoris apterus L. (Heteroptera: Pyrrhocoridae). J. Insect Behav. 13: 741-750 Go to original source...
    36. SOCHA R. & ZEMEK R. 2000b: Locomotor activity in adult Pyrrhocoris apterus (Heteroptera) in relation to sex, physiological status and wing dimorphism. Physiol. Entomol. 25: 383-389 Go to original source...
    37. SOCHA R. & ZEMEK R. 2003: Wing morph-related differences in the walking pattern and dispersal in a flightless bug, Pyrrhocoris apterus (L.) (Heteroptera). Oikos 100: 35-43. Go to original source...
    38. SOCHA R., SULA J. & ZEMEK R. 1997: Feeding, drinking and digestive enzyme activities in long- and short-day females of Pyrrhocoris apterus (Heteroptera). Physiol. Entomol. 22: 161-169 Go to original source...
    39. SOCHA R., SULA J. & ZEMEK R. 1998: Feeding behaviour, digestive physiology and lipid content in macropterous females of Pyrrhocoris apterus (L.) (Heteroptera: Pyrrhocoridae). Physiol. Entomol. 23: 91-96 Go to original source...
    40. SOCHA R., KODRIK D. & ZEMEK R. 1999: Adipokinetic hormone stimulates insect locomotor activity. Naturwissenschaften 88: 85-86 Go to original source...
    41. SOCHA R., KODRIK D., SIMEK P. & PATOCKOVA M. 2004: The kind of AKH-mobilized energy substrates in insects can be predicted without a knowledge of the hormone structure. Eur. J. Entomol. 101: 29-35 Go to original source...
    42. VAN DER HORST D.J., VAN MARREWIJK W.J.A., VULLINGS H.G.B. & DIEDEREN H.B. 1999: Metabolic neurohormones: release, signal transduction and physiological responses of adipokinetic hormones in insects. Eur. J. Entomol. 96: 299-308
    43. VAN DER HORST D.J., VAN MARREWIJK W.J.A. & DIEDEREN H.B. 2001: Adipokinetic hormones of insect: release, signal transduction, and responses. Intern. Rev. Cytol . 211: 179-240 Go to original source...
    44. VAN MARREWIJK W.J.A., VAN DEN BROEK A.T.M., VAN DER HORST D.J. & BEENAKKERS A.M.T. 1984: Hypertrehalosaemic and hyperlipaemic responses to adipokinetic hormone in fifth larval instar locusts, Locusta migratoria. Insect Biochem. 14: 151-157 Go to original source...
    45. WICHER D., WALTHER C. & PENZLIN H. 1994: Neurohormone-D induces ionic current changes in cockroach central neurons. J. Comp. Physiol. (A) 174: 507-515 Go to original source...
    46. WICHER D., AGRICOLA H.J., SOHLER S., GUNDEL M., HEINEMANN S.H., WOLLWEBER L., STENGL M. & DERST C. 2006: Differential receptor activation by cockroach adipokinetic hormones produces differential effects on ion currents, neuronal activity, and locomotion. J. Neurophysiol. 95: 2314-2325 Go to original source...
    47. WITTEN J.L., SCHAFFER M.H., O'SHEA M., COOK J.C., HEMLING M.E. & RINEHART K.L.JR. 1984: Structure of two cockroach neuropeptides assigned by fast atom bombardment mass spectrometry. Biochem. Biophys. Res. Commun. 124: 350-358 Go to original source...
    48. ZOLLNER N. & KIRSCH K. 1962: Uber die quantitative Bestimmung von Lipoide (Mikromethode) mittels der vielen natuerlichen Lipoiden (allen bekannten Plasmalipoiden) gemeinsamen Sulfo-phospho-vanillin-Reaktion. Z. Ges. Exp. Medizin 135: 545-561 Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content