Eur. J. Entomol. 98 (4): 439-444, 2001 | DOI: 10.14411/eje.2001.064

Comparative dispersal and larvicidal activity of exotic and Azorean isolates of entomopathogenic nematodes against Popillia japonica (Coleoptera: Scarabaeidae)

Lawrence A. LACEY1, J. Silvino ROSA2, Nelson O. SIMOES2, Joao J. AMARAL3, Harry K. KAYA4
1 Japanese Beetle Control Project, Terceira, Açores, Portugal
2 Departamento de Biologia, Universidade dos Açores, Ponta Delgada 9502, Portugal
3 Direcçao Regional do Desenvolvimento Agrário, Vinha Brava, 9700 Angra do Heroismo, Portugal
4 Department of Nematology, University of California, Davis, CA 95616, USA

The Japanese beetle, Popillia japonica Newman, is an introduced pest on Terceira, one of nine islands in the Azorean Archipelago. Research conducted on Terceira indicates that entomopathogenic nematodes in the families Steinernematidae and Heterorhabditidae provide good to excellent control of Japanese beetle larvae, but the species that have been evaluated are not native to the Azores. An efficacious species that is native to the archipelago might provide increased capabilities for persisting and recycling in Azorean soil and weather conditions. Surveys on the islands of Terceira and Santa Maria resulted in the isolation of two Heterorhabditis strains (São Mateus and Praia Formosa) with good larvicidal activity for P. japonica. Initial bioassays conducted with Steinernema glaseri (Steiner) originally from North Carolina against P. japonica third instar larvae and pupae produced LC50 values of 3.2 × 105 infective juveniles (IJs)/m2 and 0.9 × 105 IJs/m2, respectively. Comparative bioassays of the native isolates and S. glaseri against P. japonica revealed similar larvicidal activity. The LC50s of the São Mateus and Praia Formosa isolates against third instar larvae were 3.64 × 105 and 4.44 x 105 IJs/m2, respectively. The LC50 of S. glaseri ranged from 3.2 to 5.5 × 105 IJs/m2. The higher larvicidal activity of the Azorean Heterorhabditis isolates for P. japonica indicates that native nematodes are as effective as S. glaseri. Heterorhabditid species also have demonstrated ability for persistence and apparent recycling under conditions where sustainable control of this introduced pest is needed. Studies comparing the dispersal behavior of the Heterorhabditis bacteriophora Poinar São Mateus isolate with that of S. glaseri and native and exotic strains of Steinernema carpocapsae (Weiser) revealed that the H. bacteriophora isolate demonstrated a greater propensity to disperse than other strains in the presence or absence of P. japonica larvae. In the presence of a host, a greater proportion of H. bacteriophora and S. glaseri dispersed than either of the two S. carpocapsae strains.

Keywords: Steinernema carpocapsae, Steinernema glaseri, Heterorhabditis bacteriophora, Heterorhabditidae, Steinernematidae, Japanese beetle, Popillia japonica, dispersal

Received: May 5, 2001; Revised: August 3, 2001; Accepted: September 3, 2001; Published: December 28, 2001  Show citation

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LACEY, L.A., ROSA, J.S., SIMOES, N.O., AMARAL, J.J., & KAYA, H.K. (2001). Comparative dispersal and larvicidal activity of exotic and Azorean isolates of entomopathogenic nematodes against Popillia japonica (Coleoptera: Scarabaeidae). EJE, 98(4), 439-444. doi: 10.14411/eje.2001.064
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