Eur. J. Entomol. 117: 490-503, 2020 | DOI: 10.14411/eje.2020.052

Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling speciesOriginal article

Julie LEROY1, Jean-David CHAPELIN-VISCARDI1, Guénaëlle GENSON2, Julien HARAN2, Éric PIERRE2, Jean-Claude STREITO2
1 Laboratoire d'Éco-Entomologie, 5 rue Antoine Mariotte, 45000 Orléans, France; e-mails: leroy@laboratoireecoentomologie.com, chapelinviscardi@laboratoireecoentomologie.com
2 CBGP, INRAE, CIRAD, IRD, Institut Agro, Univ Montpellier, Montpellier, France; e-mails: guenaelle.genson@inrae.fr, julien.haran@cirad.fr, eric.pierre@inrae.fr, jean-claude.streito@inrae.fr

Oulema melanopus (Linnaeus, 1758) and Oulema duftschmidi (Redtenbacher, 1874) (Coleoptera: Chrysomelidae) are two native West Palaearctic species developing on various cultivated and wild grasses. Along with O. obscura they are considered to be secondary pests of cereal crops. However, local outbreaks have been recorded recently and their status as secondary pests may evolve, especially as the use of broad-spectrum insecticides is now greatly reduced. Oulema melanopus and O. duftschmidi are considered to be sibling species. They are morphologically very close and difficult to distinguish from each other, which makes it difficult to study them. We tested the reliability of the standard barcode fragment (COI) for distinguishing between these species. A total of 92 samples of the two species, covering the majority of their natural range, was sequenced for the barcode fragment and inter- and intraspecific genetic distances were estimated. Our results confirm those of Bezdìk & Baselga (2015, Acta Entomol. Mus. Nat. Prag. 55: 273-304) in that this marker cannot differentiate between all the species of the Oulema melanopus complex, which in the Mediterranean basin contains several described and possibly some undescribed cryptic species. However, this marker may be useful in an agricultural context in areas where only O. melanopus and O. duftschmidi occur (such as in cereal crops in France) where it can be used to reliably and rapidly separate all stages of these two taxa and can therefore help in studying their ecology and dynamics.

Keywords: Coleoptera, Chrysomelidae, Oulema, cereal leaf beetles, DNA barcode, COI, identification, crop pests, Europe

Received: March 24, 2020; Revised: July 14, 2020; Accepted: July 14, 2020; Published online: December 21, 2020  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
LEROY, J., CHAPELIN-VISCARDI, J., GENSON, G., HARAN, J., PIERRE, É., & STREITO, J. (2020). Distinct barcodes for the Cereal leaf beetles Oulema melanopus and Oulema duftschmidi (Coleoptera: Chrysomelidae), two syntopical sibling species. EJE, 117, Article 490-503. https://doi.org/10.14411/eje.2020.052
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. ACTA 2016: Guide pratique de défense des cultures. ACTA éditions, Paris, 913 pp.
    2. Anglade P., Roehrich R., Maurin G. & Lecigne P. 1976: Le Léma des céréales (Oulema melanopus L.), étude de sa nuisibilité en Aquitaine. - Défense Végét. 178: 85-100.
    3. Anisimova M. & Gascuel O. 2006: Approximate likelihood-ratio test for branches: A fast, accurate, and powerful alternative. - Syst. Biol. 55: 539-552. Go to original source...
    4. Balachowsky A. & Mesnil L. 1935: Les insectes nuisibles aux plantes cultivées. Leurs mœurs, leur destruction. Établissements Busson, Paris, 1137 pp.
    5. Bechini L., Morlacchi P. & Baumgärtner J. 2013: Coinciding development of winter wheat and leaf beetles along an Alpine transect. - Acta Oecol. 47: 95-104. Go to original source...
    6. Bergsten J., Bilton D.T., Fujisawa T., Elliott M., Monaghan M. T., Balke M., Hendrich L., Geijer J., Herrmann J., Foster G. N., Ribera I., Nilsson A.N., Barraclough T.G. & Vogler A.P. 2012: The effect of geographical scale of sampling on DNA barcoding. - Syst. Biol. 61: 851-869. Go to original source...
    7. Berti N. 1989: Contribution à la faune de France. L'identité d'Oulema (O.) melanopus (L.) [Col. Chrysomelidae Criocerinae]. - Bull. Soc. Entomol. Fr. 94: 47-57. Go to original source...
    8. Bezdìk J. & Baselga A. 2015: Revision of western Palaearctic species of the Oulema melanopus group, with description of two new species from Europe (Coleoptera: Chrysomelidae: Criocerinae). - Acta Entomol. Mus. Nat. Prag. 55: 273-304.
    9. Bezdìk J. & Mlejnek R. 2016: Icones insectorum Europae centralis. Coleoptera: Megalopodidae, Orsodacnidae, Chrysomelidae: Donaciinae, Criocerinae. - Folia Heyrovskyana (B) 27: 1-63 [in Czech and English].
    10. Bezdìk J. & Schmitt M. 2017: Subfamily Criocerinae. In Löbl I. & Smetana A. (eds): Catalogue of Palaearctic Coleoptera, vol. 6, Corrigenda et Addenda. - Entomol. Bl. Col. 113: 113-135.
    11. Bonnemaison L. 1962: Les ennemis animaux des plantes cultivées et des forêts, tome II. Éditions Sep, Paris, 500 pp.
    12. Bukejs A. & Ferenca R. 2010: The first record of Oulema duftschmidi (Redtenbacher, 1874) (Coleoptera: Chrysomelidae) in the Lithuanian fauna. - Acta Zool. Lituan. 20: 229-231. Go to original source...
    13. Burke R 2012: UE. URL: http://www.arcgis.com/home/item.html?id=6d611f8d87d54227b494d4c3becef6a0 (last accessed 18 Jan. 2018).
    14. Chambon J.-P., Van Laere C., Genestier G., Pineau C. & Cocquempot C. 1983: Étude des populations d'Oulema melanopus L. et Oulema lichenis Weiss. (Coleoptères: Chrysomelidae) sur blé dans la région parisienne. - Agronomie 3: 685-690. Go to original source...
    15. Chapelin-Viscardi J.-D. & Maillet-Mezeray J. 2015: Données bio-écologiques sur deux espèces jumelles sympatriques en milieux agricoles: Oulema melanopus (L.) et O. duftschmidi (Redtenbacher) (Coleoptera, Chrysomelidae). - Bull. Mens. Soc. Linn. Lyon 84: 19-30. Go to original source...
    16. Cognato A.I. 2006: Standard percent DNA sequence difference for insects does not predict species boundaries. - J. Econ. Entomol. 99: 1037-1045. Go to original source...
    17. Cox M.L. 2000: Progress report on the Bruchidae/Chrysomelidae recording scheme. - Coleopterist 9: 65-74.
    18. Cruaud A., Jabbour-Zahab R., Genson G., Cruaud C., Couloux A., Kjellberg F., van Noort S. & Rasplus J.-Y. 2010: Laying the foundations for a new classification of Agaonidae (Hymenoptera: Chalcidoidea), a multilocus phylogenetic approach. - Cladistics 26: 359-387. Go to original source...
    19. Drummond A.J., Ashton B., Buxton S., Cheung M., Cooper A., Heled J., Kearse M., Moir R., Stones-Havas S., Sturrock S., Thierer T. & Wilson A. 2010: Geneious v5.5. URL: http://www.geneious.com.
    20. Feytaud J. 1924: Un parasite de la Miliade, Lema flavipes Suffr. - Rev. Zool. Agric. Appl. 23: 158-161.
    21. Germain J.-F., Chatot C., Meusnier I., Artige E., Rasplus J.-Y. & Cruaud A. 2013: Molecular identification of Epitrix potato flea beetles (Coleoptera: Chrysomelidae) in Europe and North America. - Bull. Entomol. Res. 103: 354-362. Go to original source...
    22. Guindon S., Dufayard J.-F., Lefort V., Anisimova M., Hordijk W. & Gascuel O. 2010: New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. - Syst. Biol. 59: 307-321. Go to original source...
    23. Guppy J.-C. & Harcourt D.G. 1978: Effects of temperature on development of the immature stages of the cereal leaf beetle, Oulema melanopus (Coleoptera: Chrysomelidae). - Can. Entomol. 110: 257-263. Go to original source...
    24. Hebert P.D.N., Cywinska A. & Ball S.L. 2003a: Biological identifications through DNA barcodes. - Proc. R. Soc. Lond. (B) 270: 313-321. Go to original source...
    25. Hebert P.D.N., Ratnasingham S. & deWaard J.R. 2003b: Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. - Proc. R. Soc. Lond. (B) 270: S96-S99. Go to original source...
    26. Hewitt G.M. 2001: Speciation, hybrid zones and phylogeography - or seeing genes in space and time. - Mol. Ecol. 10: 537-549. Go to original source...
    27. Ivanova N.V., Zemlak T.S., Hanner R.H. & Hebert P.D.N. 2007: Universal primer cocktails for fish DNA barcoding. - Mol. Ecol. Notes 7: 544-548. Go to original source...
    28. Jolivet P. 1997: Biologie des Coléoptères Chrysomélides. Société Nouvelle des Éditions Boubée, Paris, 279 pp.
    29. Kimura M. 1980: A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. - J. Mol. Evol. 16: 111-120. Go to original source...
    30. Knechtel W.K. & Manolache C.I. 1936: Observatii biologice asupra gândacului ovazului (Lema melanopa L.) în România. - Anal. Inst. Cercet. Agron. Român. 7: 186-208.
    31. Kubisz D., Kajtoch £., Mazur M.A. & Rizun V. 2012: Molecular barcoding for central-eastern European Crioceris leaf-beetles (Coleoptera: Chrysomelidae). - Centr. Eur. J. Biol. 7: 69-76. Go to original source...
    32. Kumar S., Stecher G. & Tamura K. 2016: Mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. - Mol. Biol. Evol. 33: 1870-1874. Go to original source...
    33. Labeyrie V. 1963: Sous-famille des Criocerinae. In Balachowsky A.S. 1963: Entomologie appliquée à l'agriculture. Tome 1, Coléoptères, Second volume, Phytophagoidea (suite et fin). Masson et Cie, Paris, pp. 571-592.
    34. Lefort V., Longueville J.-E. & Gascuel O. 2017: SMS: Smart model selection in PhyML 3.0. - Mol. Biol. Evol. 34: 2422-2424. Go to original source...
    35. Leroy J. & Chapelin-Viscardi J.-D. 2018: Sympatrie et syntopie de deux espèces jumelles: Oulema melanopus (L., 1758) et Oulema duftschmidi (Redtenbacher, 1874) (Coleoptera: Chrysomelidae). - Entomologiste 74: 163-169.
    36. Letunic I. & Bork P. 2016: Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. - Nucl. Acids Res. 44: W242-W245. Go to original source...
    37. Olfert O. & Weiss R.M. 2006: Impact of climate change on potential distributions and relative abundances of Oulema melanopus, Meligethes viridescens and Ceutorhynchus obstrictus in Canada. - Agric. Ecosyst. Environ. 113: 295-301. Go to original source...
    38. Pélécassis E. 1951: Le Lema melanopa L. nuisible sur les céréales en Grèce. - Ann. Inst. Phytopathol. Benaki 5: 52.
    39. Philips C.R., Herbert D.A., Kuhar T.P., Reisig D.D., Thomason W.E. & Malone S. 2011: Fifty years of cereal leaf beetle in the US: an update on its biology, management, and current research. - J. Integr. Pest Manag. 2: C1-C5. Go to original source...
    40. QGIS Development Team 2016: QGIS Geographic Information System. Open Source Geospatial Foundation Project. URL: http://qgis.osgeo.org (last accessed 18 Jan. 2018).
    41. Ratnasingham S. & Hebert P.D.N. 2007: BOLD: The Barcode of Life Data System (www.barcodinglife.org). - Mol. Ecol. Notes 7: 355-364. Go to original source...
    42. Riley E.G., Clark S.M. & Seeno T.N. 2003: Catalog of the Leaf Beetles of America North of Mexico (Coleoptera: Megalopodidae, Orsodacnidae and Chrysomelidae, Excluding Bruchinae). Coleopterists Society, Special Publication no. 1, Sacramento, CA, 290 pp.
    43. Sajó K. 1893: Das Getreidehähnchen (Lema melanopus L.). - Z. Pflanzenkrankh. 3: 129-137.
    44. Streito J.-C., Fontaine O., Atiama M., Genson G., Pierre É., Sadeyen J., Frago E. & Nibouche S. 2018: Présence sur l'île de la Réunion de deux espèces de punaises prédatrices potentiellement utilisables pour la lutte biologique: Orius naivashae et Cyrtopeltis callosus (Hemiptera, Anthocoridae et Miridae). - Bull. Soc. Entomol. Fr. 123: 1-128. Go to original source...
    45. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. & Higgins D.G. 1997: The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. - Nucl. Acids Res. 25: 4876-4882. Go to original source...
    46. UMS 2414 RIATE 2018: GREAT (Generalised Representation for European Areas and Territories). URL: http://riate.cnrs.fr/?page_id=153 (accessed 18 Jan. 2018).
    47. Urquijo P. 1940: Una plaga de Lema melanopus L. en trigos de Galicia. - Bol. Patol. Veg. Entomol. Agric. 9: 263-267.
    48. Venturi F. 1942: La Lema melanopa L. (Coleoptera: Chrysomelidae). - Redia 28: 11-88.
    49. Warcha³owski A. 2003: Chrysomelidae: The Leaf-beetles of Europe and the Mediterranean Area. Natura Optima Dux Foundation, Warszawa, 656 pp.
    50. White R.E. 1981: Homonymy in Word Species Group Names of Criocerinae (Coleoptera: Chrysomelidae). U.S. Department of Agriculture, Technical Bulletin 1629, 69 pp.

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content