Eur. J. Entomol. 116: 477-485, 2019 | DOI: 10.14411/eje.2019.049

Effects of the juvenile hormone mimic NC-184 on the development of the reproductive organs and mating behaviour of nymphs of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae)Original article

Satoshi HIROYOSHI1, Elizabeth KOKWARO2, Sai METTUPALLI3, Takayuki MITSUNAGA4, Shigemi YAGI1, Gadi V.P. REDDY3,5,*
1 Department of Chemical Ecology, International Centre of Insect Physiology and Ecology (ICIPE), Nairobi, Kenya; e-mail: satoshi_hiroyoshi@yahoo.co.jp
2 Department of Zoology, Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya; e-mail: elizkokwaro@yahoo.com
3 Montana State University, Western Triangle Agricultural Research Center, 9546 Old Shelby Rd, PO Box 656, Conrad, MT, USA, e-mail: mrsai2002@gmail.com
4 Central Region Agricultural Research Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan; e-mail: aeiou@affrc.go.jp
5 USDA-ARS-Southern Insect Pest Management Research Unit, 141 Experiment Station Road, P.O. Box 346, Stoneville, MS 38776, USA; e-mail: gadi.reddy@usda.gov

The insect growth regulator NC-184, a juvenile hormone mimic, prevents moulting to the adult stage in the desert locust, Schistocerca gregaria (Forskål) (Orthoptera: Acrididae). Male nymphs treated in the penultimate or final nymphal instar with NC-184 exhibit precocious mating behaviour in the final instar. We examined whether this chemical affects the development of the internal reproductive organs of crowded nymphs. In treated males, both accessory glands and seminal vesicles were underdeveloped, and no sperm was found in the seminal vesicle, whereas these organs in control individuals had greatly increased in size 10 days after treatment, when all the insects had moulted to adults. Testis size in treated males was similar to that in controls, regardless of their smaller body size due to the inhibition of moulting. Oogenesis and development of spermatheca in females treated with NC-184 continued to some degree, but no eggs matured, unlike what occurred in the control. In conclusion, treatment of S. gregaria nymphs with NC-184 resulted in changes in the reproductive organs in both sexes.

Keywords: Orthoptera, Acrididae, Schistocerca gregaria, juvenile hormone mimic, moulting, reproductive organs, oogenesis, spermatheca

Received: August 21, 2019; Accepted: November 19, 2019; Published online: December 13, 2019  Show citation

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HIROYOSHI, S., KOKWARO, E., METTUPALLI, S., MITSUNAGA, T., YAGI, S., & REDDY, G.V.P. (2019). Effects of the juvenile hormone mimic NC-184 on the development of the reproductive organs and mating behaviour of nymphs of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae). EJE, 116, Article 477-485. https://doi.org/10.14411/eje.2019.049
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