Eur. J. Entomol. 121: 54-63, 2024 | DOI: 10.14411/eje.2024.008

Functional analysis of two SfHsp90 genes in response to high temperature and insecticide stress in Spodoptera frugiperda (Lepidoptera: Noctuidae)Original article

Hong-Yun RUAN ORCID...1, Lv ZHOU ORCID...1, Lei YANG ORCID...2, Jian-Yu MENG ORCID...3, Chang-Yu ZHANG ORCID...1,*
1 Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China; e-mails: 2398835118@qq.com (H.-Y. R.), 1269560136@qq.com (L.Z.), gzuzcy1121@126.com (C.-Y. Z)
2 China Tobacco Hunan Industrial Co., Ltd, Changsha, 410007, China; e-mail: 1092518523@qq.com (L.Y.)
3 Guizhou Tobacco Science Research Institute, Guiyang 550081, China; e-mail: mengjy0417@163.com (J.-Y. M.)

Spodoptera frugiperda, a worldwide pest, can feed on 353 crops species, including corn, rice, and sorghum. It is highly adaptable to various environments. Heat shock protein 90 kDa (Hsp90) plays a crucial role in the environmental adaptation of insects. To explore the role of SfHsp90 genes coding for Hsp90 proteins in the high temperature and insecticides stress resistance of Spodoptera frugiperda, we identified the complete complementary DNA sequences of two SfHsp90s. Both of them were expressed at different developmental stages and tissues in S. frugiperda. The expression levels of the SfHsp90s were significantly upregulated when exposed to durations of extreme temperature (45°C) and lethal concentrations of emamectin benzoate (LC10 and LC20). The viability of S. frugiperda under 45°C and emamectin benzoate stresses was examined. The mortality rate of S. frugiperda was significantly increased when subjected to 45°C and emamectin benzoate after knockdown of SfHsp90s by RNAi. These results suggest that SfHsp90s are essential for the resistance of S. frugiperda to high temperature and emamectin benzoate stresses.

Keywords: Crop pest, heat shock protein 90, knockdown, RNAi, emamectin benzoate, resistance

Received: November 27, 2023; Revised: January 23, 2024; Accepted: January 23, 2024; Published online: February 20, 2024  Show citation

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RUAN, H., ZHOU, L., YANG, L., MENG, J., & ZHANG, C. (2024). Functional analysis of two SfHsp90 genes in response to high temperature and insecticide stress in Spodoptera frugiperda (Lepidoptera: Noctuidae). EJE, 121, Article 54-63. https://doi.org/10.14411/eje.2024.008
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