Eur. J. Entomol. 117: 295-301, 2020 | DOI: 10.14411/eje.2020.034

Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspectsOriginal article

Mónica Gabriela CHIRINO1,2, María Florencia FOURASTIE2,3, Néstor Daniel CENTENO1, María José BRESSA2,4
1 Laboratorio de Entomología Aplicada y Forense, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Buenos Aires, Argentina; e-mails: mchirino@unq.edu.ar, ncenteno@unq.edu.ar
2 Consejo Nacional de Investigaciones Científicas y Técnicas, C1425FQB Ciudad Autónoma de Buenos Aires, Argentina
3 Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Ciudad Autónoma de Buenos Aires, Argentina; e-mail: ffourastie@ege.fcen.uba.ar
4 Grupo de Citogenética de Insectos, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Ciudad Autónoma de Buenos Aires, Argentina; e-mail: mjbressa@ege.fcen.uba.ar

Heterochromatin may vary qualitatively, quantitatively, and in its location both in and between species. There were differences recorded in sex chromosomes, satellite sizes and location of C-bands in specimens of Lucilia sericata (Meigen, 1826) from three localities in the Buenos Aires region, Argentina (Bernal, Castelar, and Buenos Aires City). Mitotic analyses revealed the same diploid chromosome number of 2n = 10 + XY / XX (male / female) and the same size, morphology, and C-banding patterns on their autosomes. However, there are different morphotypes of X and Y chromosomes with a distinctive heterochromatin content in both arms. Four morphotypes of X (X1, X2, X3, and X4) and three of Y (Y1, Y2, and Y3) were recorded. The X metacentric and Y subtelocentric chromosomes were identified based on their very large and small sizes, respectively, and both had large heterochromatic blocks. These intraspecific differences are mainly due to quantitative variations in non-coding repetitive sequences located in the arms of both X and Y sex chromosomes. The specimens were very polymorphic both between and within the three localities sampled. Cytogenetic studies on specimens of L. sericata from the Nearctic, Palaearctic and Ethiopian regions also report differences in the morphology of the sex chromosomes and their C-banding patterns, some of which may have evolved independently. Our cytogenetic observations on L. sericata indicate that the accumulation of heterochromatin in the genome could be involved in chromosomal divergence and karyotype evolution of this species, as demonstrated in other Diptera. The polymorphic sex chromosomes are therefore important for understanding the evolution within species as well as speciation.

Keywords: Diptera, Calliphoridae, Lucilia sericata, intraspecific variation, chromosome evolution, chromosome polymorphism, constitutive heterochromatin, Argentina

Received: July 7, 2019; Revised: June 16, 2020; Accepted: June 16, 2020; Published online: July 7, 2020  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
CHIRINO, M.G., FOURASTIE, M.F., CENTENO, N.D., & BRESSA, M.J. (2020). Unusual chromosome polymorphism and heterochromatin variation in the Argentinean population of the necrophagous fly Lucilia sericata (Diptera: Calliphoridae), comparison with other populations and evolutionary aspects. EJE, 117, Article 295-301. https://doi.org/10.14411/eje.2020.034
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Acuña-Morera Y., Cortés-Bernal D., Vargas M., Segura N.A. & Bello-García F. 2011: Caracterización citogenética de Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae), cepa Bogotá, Colombia. - Rev. Cienc. Salud 9: 111-124.
    2. Agrawal U.R., Bajpai N., Kurahashi H. & Tewari R.R. 2010: Metaphase karyotypes of four species of Calliphoridae (Diptera). - Chrom. Sci. 3: 49-52.
    3. Ames C. & Turner B. 2003: Low temperature episodes in development of blowflies: implications for postmortem interval estimation. - Med. Vet. Entomol. 17: 178-186. Go to original source...
    4. Baimai V. 1998: Heterochromatin accumulation and karyotypic evolution in some dipteran insects. - Zool. Stud. 37: 75-88.
    5. Boyes J.W. 1961: Somatic chromosomes of higher Diptera: V. Interspecific and intraspecific variation in the Calliphoridae. - Can. J. Zool. 39: 549-570. Go to original source...
    6. Boyes J.W. & Shewell G.E. 1975: Cytotaxonomy of Calliphoridae (Diptera). - Genetica 45: 435-488. Go to original source...
    7. Byrd J.H. & Castner J.L. 2001: Forensic Entomology: The Utility of Arthropods in Legal Investigations. CRC Press, Boca Raton, FL, 440 pp. Go to original source...
    8. Cáceres C., Segura D.F., Vera M.T., Wornoayporn V., Cladera J.L., Teal P., Sapountzis P., Bourtzis K., Zacharopoulou A. & Robinson A.S. 2009: Incipient speciation revealed in Anastrepha fraterculus (Diptera; Tephritidae) by studies on mating compatibility, sex pheromones, hybridization, and cytology. - Biol. J. Linn. Soc. 97: 152-165. Go to original source...
    9. Centeno N.D., Maldonado M. & Oliva A. 2002: Seasonal patterns of arthropods occurring on sheltered and unsheltered pig carcasses in Buenos Aires Province (Argentina). - Foren. Sci. Int. 126: 63-70. Go to original source...
    10. Chirino M.G., Folgarait P.J., Gilbert L.E., Lanzavecchia S. & Papeschi A.G. 2009: Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques. - Genet. Mol. Biol. 32: 740-747. Go to original source...
    11. Chirino M.G., Rossi L.F., Bressa M.J., Luaces J.P. & Merani M.S. 2015: Comparative study of mitotic chromosomes in two blowflies, Lucilia sericata and L. cluvia (Diptera, Calliphoridae), by C- and G-like banding patterns and rRNA loci, and implications for karyotype evolution. - Comp. Cytogenet. 9: 103-118. Go to original source...
    12. Diakova A.V., Schepetov D.M., Oyun N.Y., Shatalkin A.I. & Galinskaya T.V. 2018: Assessing genetic and morphological variation in populations of Eastern European Lucilia sericata (Diptera: Calliphoridae). - Eur. J. Entomol. 115: 192-197. Go to original source...
    13. Di Rienzo J.A., Casanoves F., Balzarini M.G., González L., Tablada M. & Robledo C.W. 2015: Grupo InfoStat. Version 2015. FCA, Universidad Nacional de Córdoba, Argentina. URL: http://www.infostat.com.ar.
    14. El-Bassiony G.M. 2006: Cytogenetic studies of Calliphora vicina and Lucilia sericata (Diptera: Calliphoridae) from northwestern Egypt. - J. Egypt. Soc. Parasitol. 36: 23-32.
    15. Forneris N.S., Otero G., Pereyra A., Repetto G., Rabossi A., Quesada-Allué L.A. & Basso A.L. 2015: High chromosomal variation in wild horn fly Haematobia irritans (Linnaeus) (Diptera, Muscidae) populations. - Comp. Cytogen. 9: 31-50. Go to original source...
    16. Greenberg B. 1991: Flies as forensic indicators. - J. Med. Entomol. 28: 565-577. Go to original source...
    17. Grewal S.I. & Jia S. 2007: Heterochromatin revisited. - Nat. Rev. Genet. 8: 35-46. Go to original source...
    18. He L.P., Watabe H.A., Zhang Y.P. & Aotsuka T. 2003: Karyotype differentiation and reproductive isolation among natural populations of Drosophila lacertosa. - Cell Res. 13: 491-497. Go to original source...
    19. Lockwood A.P.M. 1961: 'Ringer' solutions and some notes on the physiological basis of their ionic composition. - Comp. Biochem. Physiol. 2: 241-289. Go to original source...
    20. Opletalová K., Blaizot X., Mourgeon B., Chêne Y., Creveuil C., Combemale P., Laplaud A.-L., Sohyer-Lebreuilly I. & Dompmartin A. 2012: Maggot therapy for wound debridement: a randomized multicenter trial. - Arch. Dermatol. 148: 433-438. Go to original source...
    21. Picard C.J. & Wells J.D. 2010: The population genetic structure of North American Lucilia sericata (Diptera: Calliphoridae), and the utility of genetic assignment methods for reconstruction of postmortem corpse relocation. - Foren. Sci. Int. 195: 63-67. Go to original source...
    22. Rafael M.S. & Tadei W.P. 2000: Heterochromatin variation in chromosomes of Anopheles (Nyssorhynchus) darling Root and A. (N.) nuneztovari Gabaldón (Diptera: Culicidae). - Genet. Mol. Biol. 23: 67-70. Go to original source...
    23. Reeves A. & Tear J. 2000: MicroMeasure for Windows, v. 3.3. URL: http://www.colostate.edu/Depts/Biology/MicroMeasure.
    24. Rudra M. & Bahadur M. 2013: Heterochromatin variation among the populations of Mus terricolor Blyth, 1851 (Rodentia, Muridae) chromosome type I. - Comp. Cytogenet. 7: 139-151. Go to original source...
    25. Selivon D., Perondini A.L.P. & Morgante J.S. 2005: A genetic-morphological characterization of two cryptic species of the Anastrepha fraterculus complex (Diptera: Tephritidae). - Ann. Entomol. Soc. Am. 98: 367-381. Go to original source...
    26. Siljak-Yakovlev S., Godelle B., Zoldos V., Vallès J., Garnatje T. & Hidalgo O. 2017: Evolutionary implications of heterochromatin and rDNA in chromosome number and genome size changes during dysploidy: A case study in Reichardia genus. - PLoS ONE 12(8): e0182318, 21 pp. Go to original source...
    27. Steiner C.C., Suellen J.C., Marlys L.H. & Oliver A.R. 2014: Molecular phylogeny and chromosomal evolution of Alcelaphini (Antilopinae). - J. Hered. 105: 324-333. Go to original source...
    28. Stevens J. & Wall R. 1996: Classification of the genus Lucilia (Diptera: Calliphoridae): a preliminary parsimony analysis. - J. Nat. Hist. 30: 1087-1094. Go to original source...
    29. Sumner A.T. 1972: A simple technique for demonstrating centromeric heterochromatin. - Exp. Cell Res. 75: 304-306. Go to original source...
    30. Traut W. 1976: Pachytene mapping in the female silkworm Bombyx mori L. (Lepidoptera). - Chromosoma 58: 275-284. Go to original source...
    31. Ullerich F.H. & Schöttke M. 2006: Karyotypes, constitutive heterochromatin, and genomic DNA values in the blowfly genera Chrysomya, Lucilia, and Protophormia (Diptera: Calliphoridae). - Genome 49: 584-597. Go to original source...
    32. White M.J.D. 1973: Animal Cytology and Evolution. Cambridge University Press, London, 468 pp.
    33. Whitworth T.L. 2010: Keys to the genera and species of blow flies (Diptera: Calliphoridae) of the West Indies and description of a new species of Lucilia Robineau-Desvoidy. - Zootaxa 2663: 1-35. Go to original source...
    34. Whitworth T.L. 2014: A revision of the Neotropical species of Lucilia Robineau-Desvoidy (Diptera: Calliphoridae). - Zootaxa 3810: 1-76. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content