Eur. J. Entomol. 114: 500-506, 2017 | DOI: 10.14411/eje.2017.063

Pharmacological analysis of the feeding response of codling moth (Cydia pomonella; Lepidoptera: Tortricidae) neonates to bitter compounds

Maciej A. PSZCZOLKOWSKI
Darr College of Agriculture, Missouri State University, 9740 Red Spring Road, Mountain Grove, MO 65711, USA; e-mail: MPszczolkowski@missouristate.edu

Feeding in codling moth neonate caterpillars was inhibited by 0.67 mM and 2.24 mM concentrations of denatonium benzoate. This inhibitory effect was abolished by phospholipase C inhibitor, U-73122 and the phosphodiesterase inhibitor, Rolipram. Quinine and quinidine did not have inhibitory effects at concentrations as high as 1.64 mM and 0.43 mM, respectively. The inhibitory effect of denatonium was partially reversed in the presence of the calcium ion chelator, EGTA, at concentrations ranging from 2.5 µM to 250 µM. These results indicate that transduction of the taste of denatonium in codling moth neonates relies on signalling pathways that involve phospholipase C, phosphodiesterase and calcium ion influx into cells.

Keywords: Lepidoptera, Tortricidae, Cydia pomonella, codling moth, feeding, bitter taste, quinine, quinidine, denatonium, U-73122, Rolipram, calcium

Received: May 3, 2017; Accepted: October 9, 2017; Revised: October 9, 2017; Published online: November 13, 2017Show citation

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PSZCZOLKOWSKI, M.A. (2017). Pharmacological analysis of the feeding response of codling moth (Cydia pomonella; Lepidoptera: Tortricidae) neonates to bitter compounds. Eur. J. Entomol.114(1), 2017.000. doi: 10.14411/eje.2017.063.
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