Eur. J. Entomol. 98 (4): 433-438, 2001 | 10.14411/eje.2001.063

Diel changes in adipokinetic response and walking activity of Pyrrhocoris apterus (Heteroptera) in relation to physiological status and wing dimorphism

Anna MAXOVÁ1,2, Dalibor KODRÍK1,2,*, Rostislav ZEMEK1, Radomír SOCHA1
1 Institute of Entomology, Academy of Sciences, Branišovská 31, České Budějovice, 370 05, Czech Republic
2 Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, České Budějovice, 370 05, Czech Republic

Three experimental groups of adult females of Pyrrhocoris apterus (reproductive brachypterous, diapausing brachypterous, and macropterous with non-diapause type of reproductive arrest) were analysed for their dose dependency of adipokinetic response, sensitivity of the fat body to Pya-AKH and locomotor activity during a 24 hour cycle. The greatest adipokinetic response (lipid elevation after hormonal treatment) was observed in macropterous, lower in diapausing brachypterous and the lowest in reproductive brachypterous females. Macropterous females also exhibited the highest activity of walking, while the lowest locomotor activity was found in diapausing brachypters. Diel changes of adipokinetic response were recorded in all three experimental groups, with maximal response occurring during the day at 6-7 h after the onset of light. On the contrary, the minimal adipokinetic response was found in the night and early after the onset of light. No significant differences in diel rhythms were observed between groups exposed to various day lengths. Diel changes in locomotor activity were found only in reproductive brachypterous females and females of macropterous morph, and showed a diurnal rhythm with only one peak of activity during a 24 hour cycle. In the latter, the rhythmic pattern of walking activity was positively correlated with diel changes of adipokinetic response. This indicates an important role of Pya-AKH in dispersal strategy of macropterous females of this heteropteran.

Keywords: Pyrrhocoris apterus, Pya-AKH, adipokinetic response, walking activity, diurnal rhythm, diapause, wing polymorphism

Received: June 8, 2001; Accepted: October 23, 2001; Published: December 28, 2001


References

  1. Cymborowski B. 1983: Daily changes in synthesis and accumulation of neurosecretion in the brain of house cricket. J. Int. Cycle Res. 14: 111-116 Go to original source...
  2. Das S., Meier O.W. & Woodring J. 1993: Diel rhythms of adipokinetic hormone, fat body response and haemolymph lipid and sugar levels in the house cricket. Physiol. Entomol. 18: 233-239 Go to original source...
  3. Gaede G. 1999: Presence of an adipokinetic peptide in the corpus cardiacum of Dermaptera but not in the neurohaemal aorta, and chemical and functional identification of the peptide. Physiol. Entomol. 24: 327-332 Go to original source...
  4. Gaede G., Hoffmann K.H. & Spring J.H. 1997: Hormonal regulation in insects: facts, gaps, and future directions. Physiol. Rev. 77: 963-1032
  5. Holwerda D.A., Van Doorn J. & Beenakkers A.M.T. 1977: Characterization of the adipokinetic and hypertrehalosaemic substances from the locust corpus cardiacum. Insect Biochem. 7: 151-157 Go to original source...
  6. Kodrik D. & Socha R. 1999: A Mediterranean population of Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) exhibits wing morph-related differences in adipokinetic response. Eur. J. Entomol. 96: 327-330
  7. Kodrik D., Socha R., Simek P., Zemek R. & Goldsworthy G.J. 2000: A novel member of the AKH/RPCH family that stimulates locomotor activity in the firebug, Pyrrhocoris apterus (Heteroptera). Insect Biochem. Mol. Biol. 30: 489-498 Go to original source...
  8. Lee H.-J. & Wu Y.-L. 1994: Mating effect on the feeding and locomotion of the German cockroach, Blattella germanica. Physiol. Entomol. 19: 39-45 Go to original source...
  9. Lorenz M.W., Roland K., Woodring J., Hoffmann K.H. & Gaede G. 1999: Hypertrehalosaemic peptides in the honeybee (Apis mellifera): purification, identification and function. J. Insect Physiol. 45: 647-653 Go to original source...
  10. Mayer R.J. & Candy D.J. 1969: Control of haemolymph lipid concentration during locust flight: an adipokinetic hormone from the corpora cardiaca. J. Insect Physiol. 15: 611-620 Go to original source...
  11. Mullins D.E. 1985: Chemistry and physiology of the haemolymph. In Kerkut G.A. & Gilbert L.I. (eds): Comprehensive Insect Physiology Biochemistry and Pharmacology, Vol. 3. Pergamon Press, Oxford, pp. 355-400
  12. Saunders D.S. 1982: Insect Clocks. 2nd ed. Pergamon Press, New York, 279 pp
  13. Schmidt G.H., Scheer A. & Melber A. 1992: Untersuchungen zur Tagesrhytmik im Lauf- und Nahrungssuchverhalten bei Baumwollwanzen (Dysdercus intermedius Dist.) (Insecta: Heteroptera). Zool. Jb. Physiol. 96: 481-509
  14. Socha R. & Kodrik D. 1999: Differences in adipokinetic response of Pyrrhocoris apterus (Heteroptera) in relation to wing dimorphism and diapause. Physiol. Entomol. 24: 278- 284 Go to original source...
  15. Socha R. & Sula J. 1996: Differences in haemolymph proteins in relation to diapause and wing dimorphism in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae). J. Comp. Physiol. 166B: 382-376
  16. Socha R. & Zemek R. 2000a: Wing movement behavior in long- and short- winged morphs of the flightless bug Pyrrhocoris apterus L. (Heteroptera: Pyrrhocoridae). J. Insect Behav. 13: 741-750 Go to original source...
  17. Socha R. & Zemek R. 2000b: Locomotor activity in adult Pyrrhocoris apterus (Heteroptera) in relation to sex, physiological status and wing dimorphism. Physiol. Entomol. 25: 383-389 Go to original source...
  18. Socha R., Kodrik D. & Zemek R. 1999a: Adipokinetic hormone stimulates insect locomotor activity. Naturwissenschaften 88: 85-86
  19. Socha R., Kodrik D. & Zemek R. 1999b: Stimulation of locomotion in Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) is wing-morph independent and correlated with lipid mobilization by adipokinetic hormone. Eur. J. Entomol. 96: 459-461
  20. Socha R., Sula J. & Zemek R. 1997: Feeding, drinking an digestive enzyme activities in long- and short- day females of Pyrrhocoris apterus (Heteroptera). Physiol. Entomol. 22: 161- 169 Go to original source...
  21. Socha R., Sula J. & Zemek R. 1998: Feeding behaviour, digestive physiology and lipid content in macropterous females of Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae). Physiol. Entomol. 22: 91-96 Go to original source...
  22. Sula J., Kodrik D. & Socha R. 1995: Hexameric heamolymph protein related to adult diapause in the red firebug, Pyrrhocoris apterus (L.) (Heteroptera). J. Insect Physiol. 41: 793-800 Go to original source...
  23. Van Marrewijk W.J.A., Van den Broek A.T.M., Van der Horst D.J. & Beenakkers A.M.T. 1984: Hypertrehalosaemic and hyperlipaemic responses to adipokinetic hormone in fifth larval instar locust, Locusta migratoria. Insect Biochem. 14: 151-157 Go to original source...
  24. Zoellner N. & Kirsch K. 1962: Uber die quantitative Bestimmung von Lipoiden (Mikromethode) mittels der vielen natuerlichen Lipoiden (allen bekannten Plasmalipoiden) gemeinsamen Sulfophosphovanillin-Reaktion. Z. Gesamte Exp. Med. 135: 545-561 Go to original source...